性别对皮肤屏障障碍模型小鼠表皮厚度及丝聚合蛋白表达的影响
Gender Differences in Epidermis Thickness and Filaggrin Expression of Mice Skin Barrier Dysfunction Models

中文摘要：目的 探索性别对小鼠皮肤屏障功能的影响。方法 用丙酮-乙醚法和胶带粘贴法复制皮肤屏障功能障碍模型、观察性别对皮肤屏障障碍模型小鼠的表皮厚度和丝聚合蛋白(FLG)表达的影响。结果 与正常对照组相比,丙酮-乙醚组和胶带粘贴组小鼠表皮厚度增宽( P<0.05);正常对照组雄鼠与雌鼠相比,表皮厚度无差异;而丙酮-乙醚组和胶带粘贴组雌鼠表皮厚度均较同组雄鼠增加( P<0.05)。丙酮-乙醚组和胶带粘贴组与正常对照组相比,FLG蛋白表达水平降低( P<0.05),同组雄鼠表达水平均低于雌鼠( P<0.05)。结论 皮肤机械屏障功能被破坏后,小鼠的表皮厚度变化存在性别差异,而FLG蛋白表达在正常或是皮肤屏障障碍小鼠中的性别差异均有统计学意义,提示在研究小鼠皮肤屏障障碍相关疾病的发病机制和治疗措施时应重视性别差异。
英文摘要：Objective To investigate whether genders would affect mice skin barrier dysfunction models. Methods The mice skin barrier dysfunction models were constructed by the acetone-ether method and repeated tape stripping. The epidermis thickness and Filaggrin (FLG) expression of mice skin were measured by hematoxylin and eosin staining and western-blot. Results The result of morphological analysis showed that the epidermis thickness of model mice were thicker than normal control group ( P<0.05). There was no significant difference between the males and females in normal group, whereas the epidermis were thicker in the females of both model groups than the males in the same group ( P<0.05). Epidermal FLG expression was decreased in both model groups ( P<0.05), additionally, the expression levels of model mice FLG were higher in the female in all groups ( P<0.05). Conclusion There were gender differences of skin thickness in skin barrier dysfunction models. In both normal and barrier dysfunction model groups, the FLG expression showed a gender bias, and this suggested that we should pay more attention on the gender differences on the study of pathogenesis and treatment of disease with skin barrier dysfunction. 2016,(1): 105-108 收稿日期：2015-06-10 DOI：10.11984/j.issn.1000-7083.20150207 分类号：Q95-33 基金项目：云南省科技厅应用基础研究计划项目(No.2011FZ15,2013FB056); 云南省教育厅科学研究基金重点项目(No. 2013Z004); 国家中医药管理局重点学科"中西医结合基础" 作者简介：孙晓菲(1979-), 女, 讲师, 研究方向为药理学, E-mail:xiaofeisun@163.com *通讯作者：解宇环, E-mail:kmkamma@163.com 参考文献： 李钦, 王妍, 刘晓青, 等. 2008. 小鼠皮肤机械屏障功能障碍瘙痒模型的研究[J]. 云南中医中药杂志, 29(10): 51-53. 刘玮. 2008. 皮肤屏障功能解析[J]. 中国皮肤性病学杂志, 22(12): 758-761. Mona Man, 吕成志, 辛淑君, 等. 2008. 表皮通透屏障功能与表皮CD44表达关系的研究[J]. 临床皮肤科杂志, 37(7): 426-427. 田燕, 刘玮. 2013. 皮肤屏障[J]. 实用皮肤病学杂志, (6): 346-348. Azzi L, E1-Alfy M, Martel C, et al. 2005. Gender differences in mouse skin morphology and specific effects of sex steroids and dehydroepiandrosterone[J]. Journal of lnvestigative Dermatology, 124(1):22-27. Dang NN, Pang SG, Song HY, et al. 2015. Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response[J/OL]. Brazilian Journal of Medical and Biological