家鸡钠尿肽受体B基因的克隆和组织表达分析
Molecular Cloning and Tissue Expression of Chicken Natriuretic Peptide Receptors B Gene

中文摘要：钠尿肽受体B（NPR-B）作为C-型钠尿肽（CNP）的选择性受体，可介导CNP的多种生理效应，包括调控垂体激素释放、维持心血管系统稳定、调节神经系统发育、调控细胞增殖及促进骨骼生长等。本研究以家鸡 Gallus gallus domesticus为模型，首次报道了 NPR-B基因的全长cDNA，并检测其在成体家鸡中的组织表达图谱。结果显示：家鸡 NPR-B基因cDNA全长为3 189 bp，可编码1 062个氨基酸。家鸡NPR-B与人 Homo sapiens、大鼠 Rattus norvegicus、非洲爪蟾 Xenopus laevis和斑马鱼 Danio rerio分别具有79%、78%、73%、67%的氨基酸序列一致性；同时采用实时荧光定量PCR技术探究家鸡 NPR-B基因的组织表达分布，发现其在心脏、肌肉、中脑和垂体（头部）中表达水平较高。本研究结果为阐释CNP及其选择性受体NPR-B在家鸡中的生理功能奠定基础。
英文摘要：As a selective receptor of C-type natriuretic peptide (CNP), the natriuretic peptide receptor B (NPR-B) regulates various physiological processes, including pituitary hormone secretion, cardiovascular stability, nervous system development, cell proliferation and bone growth. In this study, the full-length cDNA of Gallus gallus domesticus NPR-B gene was reported and the tissue expression of NPR-B gene in adult chickens was detected by quantitative real-time PCR (qPCR). The results showed that the full length of chicken NPR-B gene was 3 189 bp encoding a protein of 1 062 amino acids. The amino acid sequence of NPR-B was highly homologous to that of Homo sapiens (79%), Rattus norvegicus (78%), Xenopus laevis (73%) and Danio rerio (67%). The result of qPCR showed that NPR-B gene was highly expressed in the heart, muscle, midbrain and pituitary of chicken. These findings laid a foundation for further explaining the physiological functions of CNP and NPR-B in chickens. 2018,37(6): 653-659 收稿日期：2018-05-03 分类号：Q959.7;Q78 基金项目：国家自然科学基金项目（31771375） 作者简介：李正阳(1991—),硕士,主要从事动物分子遗传学研究,E-mail:lzy1991716@outlook.com *通信作者：王亚军,E-mail:cdwyjhk@gmail.com 参考文献： 邓秋洋, 张剑南, 王亚军, 等. 2018. 家鸡似G蛋白偶联受体119基因的克隆与组织表达分析[J]. 四川动物, 37(1): 45-50. 祝国强, 莫春横, 李正阳, 等. 2017. 家鸡G蛋白偶联受体161的基因克隆、分子进化和组织表达[J]. 四川动物, 36(6): 632-638. Beavo JA, Brunton LL. 2002. Cyclic nucleotide research-still expanding after half a century[J]. Nature Reviews: Molecular Cell Biology, 3(9): 710-718. Chatelain D, Lesage J, Montel V, et al. 2003. Effect of natriuretic peptides on in vitro stimulated adrenocorticotropic hormone release and pro-opiomelanocortin mRNA expression by the fetal rat pituitary gland in late gestation[J]. Hormone Research in Paediatrics, 59(3): 142-148. Chusho H, Tamura N, Ogawa Y, et al. 2001. Dwarfism and early death in mice lacking C-type natriuretic peptide[J]. Proceedings of the National Academy of Sciences of the United States of America, 98(7): 4016-4021. Fowkes RC, Forrest-Owen W, Williams B, et al. 1999. C-type natriuretic peptide (CNP) effects on intracellular calcium [Ca 2+] i in mouse gonadotrope-derived αT3-1 cell line[J]. Regulatory Peptides, 84(1-3):