食物水限制对子午沙鼠肾和肝组织线粒体呼吸链复合物活性及相关自由基代谢的影响
The Effect of Food Water Deprivation on the Activity of Mitochondrial Respiratory Chain Complexes and Correlated Free Radical Metabolism in the Kidney and Liver Tissues of Meriones meridianus

中文摘要： 目的 观察食物水限制条件下对肾和肝组织线粒体呼吸链复合物活性的影响,研究组织线粒体能量代谢相关的响应特征。 方法 以健康成年子午沙鼠 Meriones meridianus为材料,运用分光光度法测定了食物水限制3 d、6 d、9 d后子午沙鼠肾脏与肝脏组织线粒体呼吸链复合物Ⅰ~Ⅳ活性及超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量变化。 结果 水限制胁迫可引起肾和肝组织中线粒体呼吸链4种复合物的活性明显升高( P<0.05, P<0.01),体质量逐渐降低。其中水限制3 d是其适应性反应的重要阶段,3 d时呼吸链复合物活性升高幅度较大,9 d时活性均降低,但仍高于对照组。肾组织线粒体SOD活性呈不同程度升高,肾与肝组织线粒体MDA含量在水限制下显著升高。 结论 食物水限制引起肾和肝组织线粒体呼吸链复合物活性的升高与肾对水的重吸收和肝代谢增加有关,长时间水限制诱导自由基水平升高,对代谢酶活性的维持可能产生不利的影响。
英文摘要： Objective To observe the effect of food water deprivation on the enzyme activity of mitochondrial respiratory chain complexes in kidney and liver tissues and investigate the reactive characteristics of the mitochondrial energy metabolism. Methods The adult healthy Meriones meridianus was used as animal model and the change of enzyme activity of mitochondrial respiratory chain complex, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in kidney and liver tissues were determined with spectrophotometric method after water restriction of 3, 6 and 9 days, respectively. Results Food water deprivation can significantly increase the activity of four mitochondrial respiratory chain complexes in kidney and liver tissues ( P<0.05, P<0.01), but gradually reduce the body weight. Three days post treatment was an important stage of adaptation reaction during the process of food water deprivation. The activity of respiratory chain complex increased most significantly after 3 days and all of them were reduced at Day 9, but were still higher than that of control. The activity of SOD in kidney mitochondria increased to varying degrees, and the content of MDA in kidney and liver mitochondria were significantly increased during water deprivation. Conclusion Food water deprivation can significantly increase the activity of mitochondrial respiratory chain complexes in kidney and liver tissues, and this may relate to the raise of water reabsorption in kidney and metabolism in liver. Long term water deprivation induced free radical level increase may have a negative effect on the maintenance of metabolism enzyme activity. 2015,34(2): 276-280 收稿日期：2014-7-4 DOI：10.3969/j.issn.1000-7083.2015.02.019 分类号：Q959.837 基金项目：甘肃省教育厅研究生导师科研项目(0801-06) 作者简介：杜海迪(1985—),女,硕士研究生,生物化学与分子生物学专业 *通讯作者：侯天德,E-mail:houtd@nenu.edu.cn 参考文献： 柳君泽, 高文祥. 1998. 缺氧大鼠脑线粒体的能量合成特点[J]. 第三军医大学学报, 20(6): 540. 谢颖, 钟才高, 刘新民, 等. 2011. 大鼠肝细胞线粒体提取质量评价的研究[J]. 毒理学杂志, (2): 159-162. 于燕妮, 王守立, 高勤, 等. 2001. SOD诱导剂对慢性氟中毒大鼠骨, 肝, 肾组织中自由基含量的影响[J]. 中国公共卫生, 17(4): 330-331. Frana MB, Panek AD, Eleutherio ECA. 2007. Oxidative stress and its effects during