秦岭滑蜥视网膜年龄相关的显微结构特征和胶质纤维酸性蛋白免疫组织化学定位
Age-dependent Microstructure and Immunohistochemical Localization of Glial Fibrillary Acidic Protein in the Retina of Scincella tsinlingensis

中文摘要：光学显微镜下观察了秦岭滑蜥 Scincella tsinlingensis视网膜年龄相关的显微结构特征，并对胶质纤维酸性蛋白（GFAP）免疫组织化学定位及表达强度进行了统计分析。结果显示：秦岭滑蜥视网膜由内至外共分为10层，与昼行性蜥蜴类视网膜结构特征一致。亚成体和成体视网膜总厚度较幼体明显增加，但三者神经节细胞层、外界膜以及视杆视锥层厚度之间无明显差异。不同年龄组视网膜细胞核层数无差异：外核层1~2层，神经节细胞层3~4层，内核层6~8层。视网膜GFAP免疫阳性主要定位于内界膜和神经纤维层，且神经纤维层阳性最强。GFAP阳性表达呈现成体最强、幼体较强、亚成体最弱的年龄相关性，这可能与视网膜胚后发育中的生理功能调节相关。
英文摘要：The age-dependent microscopic structure of Scincella tsinlingensis retina was observed under light microscope, and the immunohistochemical localization and expression intensity of glial fibrillary acidic protein (GFAP) were statistically analyzed. The results showed that the retina of S. tsinlingensis was divided into 10 layers from the inside to outside, and this was consistent with the structural characteristics of lizards' retina. The total thickness of sub-adult and adult retina increased significantly compared with the juvenile, however, no significant difference was observed among the thickness of ganglion cells layer, outer limiting membrane, and layer of rods and cones. There were also no significant differences in the number of retinal nucleus layers among different age groups, which were composed of 1-2 layers in the outer nuclear layer, 3-4 layers in the ganglion cells layer and 6-8 layers in the inner nuclear layer. The immunoreactivity of GFAP in the retina was mainly located in the inner limiting membrane and the nerve filament layer, and the nerve fiber layer was the most positive. The expression of GFAP in the retina showed age-related changes:adult > juvenile > subadult. Therefore, the expression of GFAP in the retina of S. tsinlingensis is related to age, and this may be involved in the regulation of physiological functions during the development of retina. 2018,37(4): 439-444 收稿日期：2018-03-28 DOI：10.11984/j.issn.1000-7083.20180103 分类号：Q954;Q959.6 基金项目：国家级大学生创新创业训练计划项目（201710118002） 作者简介：高燕燕(1993-),女,硕士研究生,研究方向:动物结构与机能,E-mail:yanyannn@yeah.net *通讯作者:杨纯,E-mail:yangchun774@163.com 参考文献： 鲍义恒, 陈云俊. 1979. 蛤蚧视网膜显微结构及其光感受细胞的亚显微结构[J]. 生物化学和生物物理学进展, 6(3):71-74. 邓海波, 谢伯林, 朱丽, 等. 2012. 兔眼钝挫伤后视网膜Müller细胞GFAP表达的变化[J]. 国际眼科杂志, 12(9):1647-1649. 柯技, 陈晓瑞, 易少华, 等. 2007. 大鼠视神经挫伤后视网膜神经节细胞形态改变及GFAP表达的变化[J]. 华中科技大学学报(医学版), 36(3):370-373. 马小梅, 计翔. 2001. 中国石龙子个体发育过程中头部两性异型和食性的变化[J]. 生态学杂志, 20(3):12-16. 孙庆艳, 梅斌, 王海涛, 等. 2004. 猫视网膜年龄相关的形态学变化[J]. 动物学研究, 25(6):538-542. 王厚华, 曲富金, 张铁峰. 1980. 两种夜间活动壁虎的视网膜光学显微镜和电子显微镜观察[J]. 动物学报, 26(1):102-103. 王丽敏, 邢向阳, 杨纯. 2015. 秦岭滑蜥排泄系统组织形态学观察[J]. 四川动物, 34(3):389-393. 王丽敏, 杨纯, 郭俐. 2016. 秦岭滑蜥消化系统组织结构及消化管嗜银细胞观察[J]. 动物学杂志, 51(4):614-622. 王丽敏. 2016. 秦岭滑蜥端脑细胞构筑及成体神经发生[D]. 临汾:山西师范大学. 王志宝. 2000.