Prominin1在C57BL/6小鼠视网膜色素上皮中的表达

目的 探究Prominin1在不同发育时期小鼠视网膜色素上皮（retinal pigment epithelium, RPE）的表达。方法 孕鼠和出生后的Prom1cre/ERT2+/Gt(ROSA)26SorCAG-ZsGreen1+转基因小鼠，腹腔注射他莫昔芬，将视网膜组织冰冻切片后，采用激光共聚焦显微镜检测Prominin1在转基因小鼠RPE中的表达；利用荧光定量PCR和免疫荧光技术检测Prominin1在野生型小鼠不同发育时期RPE中的表达。结果 对13.5d（E13.5）、15.5d（E15.5）和出生后1d（P1）小鼠的研究结果显示： Prominin1可能在胎鼠的部分RPE表达，出生后小鼠不表达。定量PCR显示： Prominin1在胎鼠RPE（E10.5、E13.5、E15.5和E17.5）表达明显高于出生后（P1和P14）（P<0.01）；在胚胎时期，Prominin1在E13.5和E15.5的RPE表达明显高于其他时期（P<0.05或P<0.01）。免疫荧光染色结果显示，Prominin1在E10.5、E13.5、E15.5和E17.5的RPE表达，而在P1不表达。结论 Prominin1在胎鼠视网膜色素上皮中表达，在E13.5和E15.5的胎鼠中尤为明显。
Objective To investigate the expression of Prominin1 in Retinal Pigment Epithelium (RPE) of mice at differential development stages. Methods Tamoxifen was intraperitoneally injected in pregnant or postnatal cre/ERT2+/CAG-ZsGreen1+ transgenic mice. The expression of Prominin1 in RPE of transgenic mice was examined by confocal laser scanning microscope with frozen section. In addition, the expression of Prominin 1 in developmental RPE of wild type mice was detected with real-time PCR and immunofluorescence. Results Prominin1was expressed in RPE of E13.5, E15.5 of transgenic mice tracer, however, not expressed in postnatal mice (P1). Real-time PCR showed that the expression of prominin1 was significantly higher in embryonic stages (E10.5, E13.5, E15.5 and E17.5) than that in postnatal stages (P＜0.05). Moreover, the expression of Prominin1 in RPE at E13.5 and E15.5 was significantly higher than that in other embryonic periods（P<0.05 or P<0.01）. Immunofluorescence showed that Prominin1 was expressed at E10.5, E13.5, E15.5 and E17.5, but not expressed at P1. Conclusion Prominin 1 is expressed in RPE at different embryonic stages of mice, especially, at E13.5 and E15.5