COPD急性发作期患者外周血IL-35、hs-CRP检测及临床意义

目的 观察慢性阻塞性肺疾病急性发作期(acute exacerbation of chronic obstructive pulmonary disease, AECOPD)患者外周血IL-35、hs-CRP水平,并探讨其临床意义。 方法 80例COPD患者分为急性发作期组(A组,n=49)和稳定期组(B组,n=31),同期健康体检者25名为对照者(C组,n=25)。ELISA法检测3组外周血清IL-35表达；检测3组外周血清hs-CRP。 结果COPD患者B组、A组外周血清IL-35表达水平分别为(4.171±1.339)ng/ml、(3.480±1.790)ng/ml,与对照组[(5.194±1.595)ng/ml]相比,差异有统计学意义(P<0.05)；B组与A组相比,差异无统计学意义(P>0.05)。A组患者血清hs-CRP水平[(61.143±30.024)mg/L]明显高于B组和C组[(12.161±4.495mg/L、(1.075±0.855)mg/L],差异有统计学意义(P<0.05)。A组外周血清IL-35与hs-CRP呈负相关性(r=-0.349,P=0.014),B组无负相关(r=-0.237,P=0.199)。 结论 AECOPD患者外周血清IL-35低表达及hs-CRP高表达,两者联合检测可作为临床诊断AECOPD的辅助指标。
Objective To investigate serum IL-35 and hs-CRP level in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD), and their clinical significance. Methods Eighty patients with COPD, including 49 cases of acute exacerbation (group A) and 31 cases of stable disease (group B), and 25 healthy subjects (group C) were recruited in the study. Serum levels of IL-35 and hs-CRP were determined by ELISA. Results Compared to control group [(5.194±1.595)ng/ml], serum IL-35 levels in group B and A were significantly lower [(4.171±1.339)ng/ml and(3.480±1.790)ng/ml; P<0.05], while there was no significant difference between group B and group A (P>0.05). Serum Hs-CRP levels in group A were significantly higher than those in group B and group C (61.143±30.024mg/L vs. 12.161±4.495mg/L and 1.075±0.855mg/L, P<0.05). The serum IL-35 was negatively correlated with hs-CRP (r=-0.349, P=0.014) in group A, but not in group B (r=-0.237, P=0.199). Conclusion The low serum IL-35 level and high hs-CRP level in AECOPD patients may be used as an indicator for clinical diagnosis