miR-182在三阴性乳腺癌中的表达及意义

目的探讨miR-182在三阴性乳腺癌中的表达及意义。方法 收集30例石蜡包埋的三阴性乳腺癌患者的癌组织和癌旁组织标本,并收集患者的基本资料。RT-PCR测定miR-182的相对表达,分析miR-182在TNBC中的表达与肿瘤的大小、分级、淋巴结转移、远处转移、TNM分期、无疾病生存率、总生存率等的相关性。结果RT-PCR结果提示,癌组织中miR-182表达为癌旁组织的8.6倍,差异有统计学意义(P＜0.001)。miR-182在淋巴结阳性及TNM分期Ⅲ期中表达高于淋巴结阴性(Z=-3.454,P=0.001)及TNM(Ⅰ+Ⅱ)期者(Z=-3.946,P=0.000),差异有统计学意义；COX回归多因素分析显示,miR-182高表达(HR=0.107,95％CI:0.018～0.643,P=0.015)、淋巴结阳性(HR=0.189,95％CI:0.020～1.757,P=0.016)、脉管瘤栓(HR=0.190,95％CI:0.020～1.826,P=0.008)、Ki67≥14％(HR=0.182,95％CI:0.041～0.801,P=0.024)、TNM分期(Ⅲ期)(HR=0.501,95％CI:0.044～5.741,P=0.001)是独立的预后因子。结论 miR-182在三阴性乳腺癌中表达上调,与淋巴结转移及临床分期相关,miR-182高表达是三阴性乳腺癌独立的预后因素之一,与预后差相关。
Objective To investigate the expression of microRNA-182(miR-182) in triple-negative breast cancer(TNBC) and its clinicopathological significance. Methods Thirty patients with triple-negative breast cancer underwent mastectomy, and the surgical specimens were collected. Quantitative Real-Time polymerase chain reaction(RT-PCR) was used to detect the expression of miR-182 in cancer tissues and pericancerous tissue. And the relationship between miR-182 expression in TNBC cancer tissues and clinicopathological characteristics, including tumor size, grade, lymph node metastasis, distant metastases, TNM staging, disease-free survival, distant metastasis-free survival, overall survival were analyzed. ResultsRT-PCR results indicated that the expression of miR-182 in cancer tissue was 8.6 times higher as that in pericancerous tissue (P＜0.001). In cases of lymph node positive and TNM stage Ⅲ, the expression of miR-182 was higher than those of lymph node negative(Z=-3.454,P=0.001) and TNM stage(Ⅰ+Ⅱ)(Z=-3.946,P=0.000).Cox proportional hazards risk analysis indicated that the high expression of miR-182(HR=0.107,5% CI:0.018-0.643,P=0.015), positive lymph nodes(HR=0.189,5% CI:0.020-1.757,P=0.016), lymphovascular invasion(HR=0.190,5% CI:0.020-1.826,P=0.008), Ki67≥14％(HR=0.182,5% CI:0.041-0.801,P=0.024), TNM staging Ⅲ(HR=0.501,5% CI:0.044-5.741,P=0.001) were the independent prognostic factors for TNBC. Conclusion The expression of miR-182 is up-regulated in TNBC, which is correlated with positive regional lymph nodes metastasis and advanced TNM stage. The high expression of miR-182 is one of the independent prognostic factors of TNBC, associated with poor prognosis