E26转录因子和VEGF在卵巢上皮性肿瘤中的表达及意义

目的 探讨E26转录因子(E26 transformation specific-1, Ets-1)及血管内皮生长因子(vascular endothelial growth factor, VEGF)在卵巢上皮性肿瘤中的表达及意义。方法 在55例卵巢上皮性肿瘤以及12例正常卵巢组织中,采用免疫组化SP法检测其中Ets-1及VEGF蛋白的表达；原位杂交法检测组织中Ets-1mRNA的表达。结果Ets-1及VEGF在卵巢上皮性癌中的表达显著高于正常、良性及交界组(P<0.01)。FIGO Ⅲ-Ⅳ期组中Ets-1以及VEGF蛋白的表达均高于FIGO Ⅰ-Ⅱ早期组(P<0.05)；肿瘤细胞低分化组中Ets-1的表达明显高于中高分化组(P<0.01)；有淋巴结转移组中两者表达高于无淋巴结转移组(P<0.05)；Ets-1蛋白和m RNA的表达显著相关(rs为0.609,P<0.01)；肿瘤组织中Ets-1和VEGF的表达密切相关(rs=0.288,P<0.05)。结论 Ets-1和VEGF是卵巢上皮性肿瘤中重要的促血管生成因子,与肿瘤的发展转移及患者的预后关系密切。
Objective To examine the expression of E26 transformation specific-1(Ets-1) and vascular endothelial growth factor(VEGF) in epithelial ovarian neoplasm, and its clinicopathological significance. Methods The expression of Ets-1 and VEGF proteins were detected by immun-ohistochemistry, and the expression of Ets-1mRNA was detected by in situ hybridization in 55 tissue samples of epithelial ovarian neoplasms and 12 samples of pericancerous ovarian tissue. ResultsSerous cystadenocarcinoma had higher expression of both Ets-1 and VEGF than normal ovarian tissue, serous cystadenoma and borderline serous cystadenoma(P <0.01). The expression of Ets-1 and VEGF in ovarian carcinomaⅠ-Ⅱ stage was lower than that in carcinoma Ⅲ-Ⅳ stage(P<0.05); expression of Ets-1 in low histological grade ovarian carcinoma was higher than that in middle and high histological grade carcinoma(P<0.01); the expression of Ets-1 and VEGF in ovarian carcinoma without lymph nodes metastasis was lower than that in those with metastases(P<0.05). The expression of Ets-1 mRNA was positively correlated with expression of Ets-1 and VEGF proteins(rs＝0.609, rs＝0.288, respectively, both P<0.05). Conclusion The results indicate that Ets-1 and VEGF may be involved in the development and metastases of epithelial ovarian neoplasms