粗糙钛表面纳米掺锶羟基磷灰石涂层对 BMSCs成骨分化的影响

目的 研究粗糙钛表面纳米掺锶羟基磷灰石涂层对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMSCs)增殖、成骨分化的影响。方法采用电化学沉积技术在粗糙钛表面沉积薄层纳米掺锶羟基磷灰石、纳米羟基磷灰石,用场发射扫描电子显微镜观察其表征,分析锶元素修饰的纳米羟基磷灰石涂层对大鼠BMSCs增殖、碱性磷酸酶活性、矿化结节形成和成骨相关蛋白骨钙素分泌的影响。结果 纳米掺锶羟基磷灰石涂层和纳米羟基磷灰石涂层不影响细胞增殖,与目前临床广泛应用的金属钛种植体粗糙表面一样具有良好的生物相容性。相较于纳米羟基磷灰石涂层和粗糙组,纳米掺锶羟基磷灰石涂层可增强BMSCs碱性磷酸酶活性,促进矿化结节形成,提高细胞骨钙素的分泌。纳米羟基磷灰石涂层亦表现了比粗糙组更好的生物活性。结论 应用电化学沉积技术进行纳米掺锶羟基磷灰石涂层的制备,可以促进BMSCs成骨分化、种植体周围新骨早期形成,提高种植体骨组织结合率。
Objective To investigate osteogenesis of bone marrow mesenchymal stem cells(BMSCs) on strontium-substituted nano-hydroxyapatite(Sr-HA) coated roughened titanium surface. MethodsSr-HA coating and HA coating were fabricated on roughened titanium surfaces by electrochemical deposition technique and characterized by field emission scanning electron microscope(FESM). BMSCs were cultured on Sr-HA coating, HA coating and roughened titanium surfaces respectively. Cell proliferation, alkaline phosphatase(ALP) activity, mineralized nodules formation and cell osteocalcin(OC) secretion were measured. ResultsElectrochemically deposited Sr-HA coating and HA coating titanium surface had no effect on the proliferation of BMSCs and had good biocompatibility. BMSCs cultured on Sr-HA coating surface showed increased alkaline phosphatase activity, mineralized nodules formation, and cell osteocalcin secretion compared with the other two groups. Cells cultured on HA coating surface also showed increased biological activity compared with the roughened surface. ConclusionSr-HA coated titanium surfaces by electrochemical deposition can promote osteogenesis of BMSCs in vitro and have the potential to shorten bone healing period and enhance implant osseointegration