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-  2015 

不同雌孕激素水平对子宫内膜上皮细胞Claudin-4表达的意义

DOI: 10.16118/j.1008-0392.2015.02.011

Keywords: 雌激素 孕激素 紧密连接 子宫内膜
estrogen progesterone closely connection endometrial

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Abstract:

目的 观察不同雌、孕激素水平对子宫内膜上皮细胞Claudin-4表达的影响。方法RL95-2细胞系培养于含10%胎牛血清培养基中,在培养基中加入雌、孕激素,一组孕酮的浓度恒定为0.025μmol/L,雌二醇终浓度分别为1、0.5、0.25、0.125、0.0625和0nmol/L;另一组雌二醇的浓度恒定为0.25nmol/L,孕酮终浓度分别为0.1、0.05、0.025、0.0125、0.00625和0μmol/L。两组分别培养24、48和72h,RT-PCR和Western印迹法检测RL95-2细胞的Claudin-4基因和蛋白表达。结果在雌激素恒定的条件下,RL95-2细胞的Claudin-4基因和蛋白表达随着孕激素水平的升高而升高(P<0.01),随着培养时间的延长,其表达Claudin-4基因和蛋白水平升高(P<0.01)。在孕激素恒定的条件下,RL95-2细胞的Claudin-4基因和蛋白表达随着雌激素水平的升高而降低(P<0.01),随着培养时间的延长,其表达Claudin-4基因和蛋白水平降低(P<0.01)。结论 Claudin-4在子宫内膜细胞的表达水平与雌孕激素的水平具有密切的联系,Claudin-4可能参与子宫容受性的形成和胚泡在子宫的着床有关。
Objective To investigate the association of estrogen and progesterone levels with Claudin-4 expression in endometrial epithelial cells. MethodsEndometrial epithelial RL95-2 cells were cultured in 10% FBS-containing medium, and different concentrations of estrogen and progesterone were added into the culture medium. When the concentration of progesterone maintained at 0.025μmol/L, the final concentrations of estradiol were 1,0.5,0.25,0.125,0.0625 and 0nmol/L; or when the estradiol concentration maintained at 0.25nmol/L, the final concentration of progesterone were 0.1,0.05,0.025,0.0125,0.00625 and 0μmol/L, RL95-2 cells were cultured for 24h, 48h and 72h, respectively, the expression of Claudin-4 mRNA and protein was detected by RT-PCR and Western blotting. ResultsUnder constant concentration of estrogen, the expression of Claudin-4 mRNA and protein in RL95-2 cells was increased as the progesterone levels increased or as the culture time was elongated(P <0.01). Under constant conditions of progesterone, the expression of Claudin-4 mRNA and protein was decreased in RL95-2 cells as estrogen levels increased or as the culture time was elongated(P <0.01). ConclusionThe expression of Claudin-4 in endometrial epithelial cells is closely correlated with estrogen and progesterone levels, indicating that Claudin-4 may be involved in the formation of uterine receptivity and embryo implantation

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