人精浆中胎盘生长因子表达与少弱精子症临床相关性分析

目的 探究人精浆中胎盘生长因子（PlGF）的表达水平及其与精子质量的相关性，以及其在少弱精子症和无精子症中的临床意义。方法 选择2014年9月至11月于第二军医大学长海医院生殖医学中心行精液检测的88例患者，根据检测结果分为3组：10例精液正常者、68例少弱精子症患者以及10例无精子症患者。对其中5例精液正常者和5例少弱精子症患者的精浆样本进行细胞因子相关蛋白质芯片检测。利用ELISA法检测精浆中PlGF的含量，运用Pearson相关性分析研究精浆中PlGF含量与精液中精子浓度和活力、患者年龄的相关性。结果 精液正常组患者精浆中PlGF含量高于少弱精子症组和无精子症组（P<0.05）。精浆中PlGF含量与精子的浓度和活力均存在相关性（r=0.362、0.253，P均<0.05），与患者的年龄无明显相关性。结论 精浆中PlGF含量与精子的浓度存在相关性，临床上可为少弱精子症和无精症的诊断和治疗提供参考。
Objective To explore the correlation between placental growth factor (PlGF) expression and sperm quality in seminal plasma, and to evaluate its clinical significance in patients with oligoasthenospermia and azoospermia. Methods A total of 88 patients undergoing seminal fluid detection were enrolled from the Department of Reproductive Medicine Center of Changhai Hospital of Second Military Medical University from Sep. 2014 to Nov. 2014. The patients were divided into three groups according to the test results:ten cases with normal semen (normal group), 68 cases with oligoasthenospermia (oligoasthenospermia group), and ten cases with azoospermia (azoospermia group). Semen samples from five patients with normal semen and 5 patients with oligoasthenospermia were collected and assayed for cytokine-related protein microarray. The contents of PlGF in seminal plasma were detected by ELISA. The correlations between PlGF concentration and the sperm concentration and activity in seminal plasma and the age of patients were analyzed by Pearson correlation analysis. Results The contents of PlGF in seminal plasma in the normal group were significantly higher than that in the oligoasthenospermia and azoospermia groups (P<0.05). The level of PlGF in seminal plasma was related to the concentration and activity of sperm (r=0.362, 0.253; P<0.05), but not to the age of patients. Conclusion The level of PlGF in seminal plasma is correlated with the sperm concentration, providing reference for diagnosis and treatment of oligoasthenospermia and azoospermia in clinic