一种用于前列腺癌细胞体外检测生物导航纳米探针的制备

目的 制备一种用于对人前列腺癌PC3细胞进行体外检测的粒细胞靶向介导的磁性-荧光纳米探针。方法 制备以Fe3O4为核、表面包被SiO2和异硫氰酸罗丹明的具有磁性且携带红色荧光的纳米材料，将其与正常人外周血粒细胞按不同比例混合，分别孵育不同时间以检测纳米材料对粒细胞的毒性。选择最佳比例将纳米材料和粒细胞体外结合，得到粒细胞靶向介导的磁性-荧光纳米探针；将PC3细胞与正常人全血细胞分别以不同比例混合，加入上述探针，于荧光显微镜下观察探针的靶向情况。结果 在实验所用的浓度和作用时间范围内，制备的纳米探针对粒细胞存活率无明显影响。镜下可见纳米探针在PC3细胞周围富集，形成“花瓣样”结构，而全血细胞周围无探针富集。结论 本研究制备的磁性-荧光纳米探针不会对粒细胞本身产生明显毒性，利用粒细胞对肿瘤细胞的生物靶向作用可使纳米探针有效地靶向检测肿瘤细胞。
Objective To prepare a granulocyte targeting-mediated magnetic-fluorescent nanoprobe for detecting prostate cancer PC3 cells in vitro. Methods The nanometer materials with magnetic and red fluorescence, which were prepared using Fe3O4 as the core, and SiO2 and rhodamine isothiocyanate as the shell, were mixed with normal human peripheral blood granulocytes in different proportions, and co-incubated for different periods to examine the toxicity of nanometer materials to granulocytes. The best proportion was selected to combine the nanometer materials and granulocytes in vitro. Finally we obtained the granulocyte targeting-mediated magnetic-fluorescent nanoprobes. We mixed PC3 cells and normal human whole blood cells in different proportions, added the nanoprobes, and then observed the targeting situation of the nanoprobes under a fluorescence microscope. Results The nanoprobe had no obvious influence on the survival rate of granulocytes at different concentrations and action times set in this study. The nanoprobes were enriched around the PC3 cells with a "petal-like" structure, but the peripheral blood cells were not enriched by probes. Conclusion The magnetic-fluorescent nanometer materials prepared in this study have no toxicity to granulocytes, and it can effectively detect tumor cells by the biological targeting effect of granulocytes on tumor cells