雷公藤红素抑制鼻咽癌细胞的增殖、迁移及上皮间质转化

目的 研究雷公藤红素在鼻咽癌细胞增殖、迁移及上皮间质转化中的作用。方法 使用终浓度为1.8 μmol/L的雷公藤红素处理人鼻咽癌HNE1、CNE2细胞，并设二甲基亚砜（DMSO）对照组。用CCK-8法检测HNE1、CNE2细胞的增殖能力，细胞划痕实验检测细胞迁移能力，细胞克隆形成实验检测细胞的克隆形成数，细胞黏附与分离实验检测细胞的黏附、分离能力，蛋白质印迹法检测细胞上皮间质转化蛋白（E-钙黏蛋白、β-连环蛋白、N-钙黏蛋白、波形蛋白）的表达。结果 与DMSO对照组比较，采用1.8 μmol/L雷公藤红素处理24、48、72 h后，HNE1细胞的增殖能力均降低（P均<0.05），处理48、72 h后，CNE2细胞的增殖能力均降低（P均<0.05）；处理48 h后，HNE1、CNE2细胞的迁移能力均下降（P均<0.05）；处理2周后，HNE1、CNE2细胞的克隆形成数均减少（P均<0.05）；处理1 h后，HNE1、CNE2细胞的黏附率降低（P<0.05）；处理24 h后，HNE1、CNE2细胞的分离率降低（P<0.05）；处理6 h后，HNE1、CNE2细胞中E-钙黏蛋白、β-连环蛋白的表达升高（P<0.05），N-钙黏蛋白、波形蛋白的表达降低（P均<0.05）。结论 雷公藤红素可抑制鼻咽癌HNE1、CNE2细胞的增殖、迁移及上皮间质转化。
Objective To investigate the effect of celastrol on proliferation, migration and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells. Methods Human nasopharyngeal carcinoma cells HNE1 and CNE2 were treated with 1.8 μmol/L celastrol, and the cells treated with equal volume of dimethyl sulfoxide (DMSO) were used as control. The proliferation of HNE1 and CNE2 cells was detected by CCK-8 assay, the migration of cells was detected by cell scratch test, the clone formation was detected by cell colony formation assay, the adhesion and separation abilities of cells were examined by cell adhesion and separation experiments, and the expression of E-cadherin, β-catenin, N-cadherin and vimentin was detected by Western blotting analysis. Results Compared with the DMSO group, the proliferation of HNE1 cells was significantly decreased after treated with 1.8 μmol/L celastrol for 24, 48 and 72 h (all P<0.05), and the proliferation of CNE2 cells was significantly decreased for 48 and 72 h (both P<0.05); the migration ability of HNE1 and CNE2 cells was significantly decreased after treated with 1.8 μmol/L celastrol for 48 h (both P<0.05); and the clone formation of HNE1 and CNE2 cells was significantly decreased after treated with 1.8 μmol/L celastrol for two weeks (both P<0.05). The adhesion rate of HNE1 and CNE2 cells 1 h after treatment and separation rate 24 h after treatment in the celastrol groups were significantly lower than those in the corresponding DMSO group (both P<0.05). Western blotting analysis showed that the expressions of E-cadherin and β-catenin in HNE1 and CNE2 cells in the celastrol groups were significantly increased compared with the DMSO group, while the expressions of N-cadherin and vimentin were significantly decreased 6 h after treatment (all P<0.05). Conclusion Celastrol can inhibit the proliferation, migration and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells HNE1 and CNE2