氯化血红素与十二烷基-β-D-麦芽糖苷复合胶团的催化氧化性能

研究烷基糖苷类非离子表面活性剂十二烷基-β-D-麦芽糖苷（n-Dodecyl-β-D-maltopyranoside，DDM）与氯化血红素组成的仿生复合体系的催化氧化性能，用动态光散射和紫外可见吸收光谱法表征两者的胶团化行为以及DDM对氯化血红素的分散效果，采用过氧化氢作为氧化剂底物，考察两者所形成复合体系针对模型化合物酸性橙的催化氧化性能。结果表明：该催化反应的动力学过程服从米-曼氏方程，用Lineweaver-Burk作图法求得对底物酸性橙的米氏常数（Km）和最大反应速率（vmax）分别为0.1 mmol/L和6.0×10-4 mmol/（L·min）；对底物过氧化氢的Km和vmax分别为4.6 mmol/L和9.2×10-4 mmol/（L·min）；与天然过氧化物酶相比，氯化血红素-DDM复合胶团催化体系制备容易、成本低、稳定性好。
The catalytic oxidation property of bionic composites formed by hemin and nonionic surfactant n-Dodecyl-β-D-maltopyranoside (DDM)was studied. The dynamic light scattering (DLS) and UV-vis spectrophotometry were used to characterize the micellization behavior and the DDM dispersion effect on hemin. And the catalytic oxidation property of the composite system for the model compound Orange Ⅱ was studied, in which the H2O2 was used as an oxidant substrate. The results show that the catalytic reaction system obeys the Michaelis-Menten equation of enzymatic catalysis. The Michaelis-Mentenconstant(Km) and maximum velocity(vmax) of Orange Ⅱ are estimated by Lineweaver-Burk model to be 0.1 mmol/L and 6.0×10-4 mmol/(L·min), respectively. The Km and vmax of the H2O2 are 4.6 mmol/L and 9.2×10-4 mmol/(L·min). In comparison with natural peroxidases, the hemin-DDM micelles catalytic system has characteristics of easy preparation, low cost and good stability