氟对茶树花粉管抑制及其与NO关系的研究

[目的] 茶树是超富集氟的植物，本文探究氟对茶树花粉萌发率和花粉管生长的作用及其与广谱信号分子一氧化氮（NO）的关系。[方法] 以无性系茶树品种‘龙井长叶’的花粉为材料，通过不同质量浓度的氟、NO供体（DEA NONOate）和NO清除剂（cPTIO）处理，研究了不同浓度氟和NO对茶树花粉离体萌发和花粉管生长的作用，并检测了氟处理后茶树花粉管中NO相对含量和NO合酶（NOS）的相对活性。[结果] 氟显著抑制花粉的萌发率和花粉管的伸长，加入25 μmol？L-1外源NO供体和氟共处理，增强了氟的抑制效应，而加入200 μmol？L-1 NO清除剂和氟共处理能够有效缓解氟的抑制效应。此外，氟处理促进茶树花粉管NO释放并伴随着NOS活性的提高。[结论] 氟抑制茶树花粉萌发和花粉管伸长与其诱导NO释放有关，且氟诱导的NO释放部分依赖于NOS的活性。
[Objectives] Fluorine can accumulate in tea plants (Camellia sinensis), but the machenism remains unclear. Here we detect the effect of fluorine on the pollen germination and pollen tube growth of C. sinenesis and its relationship with the broad-spectrum signal molecule nitric oxide (NO).[Methods] Pollen grains from a tea plant cultivar ‘Longjingchangye’ were used as the material. The effects of different concentrations of fluorine, NO donor (DEA NONOate)and NO scavenger (cPTIO)on pollen germination and pollen tube growth were detected by statistics methods. The relative NO content and NO synthase (NOS)activity in pollen tube of tea plant were measured by means of plant physiology and related commercial kits.[Results] The pollen germination rate and pollen tube elongation were significantly inhibited by fluorine. The inhibitory effect of fluorine was enhanced by the concentration of 25 μmol？L-1 exogenous NO donor and fluoride treatments, while the concentration of 200 μmol？L-1 NO scavenger effectively alleviated the inhibitory effect of fluorine. In addition, the NO production in pollen tubes was promoted by fluorine treatment along with the increase in NOS activity.[Conclusions] NO production was induced during fluorine inhibiting the pollen germination and pollen tube elongation of C. sinensis, and the induction of NO by fluorine was partly dependent on NOS activity