美藤果籽粕多酚的提取及其抗氧化活性测定

采用超声波辅助乙醇法提取美藤果籽粕多酚,通过单因素和正交试验法确定了美藤果籽粕多酚的较佳提取条件。结果显示,较佳提取条件为乙醇体积分数80%、料液比1:10、提取时间40 min和提取次数3次,在此条件下,美藤果籽粕多酚含量为0.623 mg/g。采用DPPH自由基清除活性测定方法评价了美藤果籽粕多酚的抗氧化活性,其清除DPPH自由基的SC50值为159.303 μg/mL。
Polyphenols were extracted from sacha inchi seed cake by ultrasonic-assisted ethanol method.The optimum extraction condition was investigated by single factor and orthogonal tests.The results showed that the optimum extraction condition was obtained when the ethanol volume fraction was 80%,the solid-liquid ratio was 1:10,40 min extraction time and repeated 3 times.Under this condition,the polyphenol yield was 0.623 mg/g. The antioxidant activity of the obtained polyphenols was evaluated by DPPH radical scavenging activity test, and its SC50 value against DPPH free radical was 159.303 μg/mL