抗松材线虫病赤松胚性愈伤组织的诱导及增殖

以抗松材线虫病赤松未成熟合子胚为外植体,研究了合子胚发育阶段、激素种类及浓度以及碳源等对胚性愈伤组织诱导的影响。结果表明:发育至2～4阶段的合子胚为胚性愈伤组织诱导的适宜外植体,2,4-D和6-BA是最优激素组合,麦芽糖比蔗糖、葡萄糖更有利于胚性愈伤组织的诱导。抗松材线虫病赤松胚性愈伤组织诱导率总体较低,以DCR为基本培养基,添加2 mg /L 2,4-D和1 mg /L 6-BA时诱导率最高为5.0%,诱导的胚性愈伤组织在增殖培养基上能够稳定增殖。
Embryogenic callus was induced from immature embryos of nematode-resistant Pinus densiflora. Factors affecting callus initiation were investigated. The results showed that successful initiation of embryogenic callus depended heavily on embryo developmental stage, the combination of auxin and cytokinins, and carbon source. The initiation of embryogenic callus worked best when immature mega gametophytes developed at stage 2-4 were used as starting material. The optimal combination of hormone was 2 mg /L 2,4-D and 1 mg /L 6-BA with the induction rate of 5%. Maltose was better than sucrose and glucose. Further proliferation of embryogenic callus was achieved by transfering explants to a medium with reduced levels of plant growth regulators