基质、插穗及促根剂对喜树扦插生根的影响

为探索不同处理对喜树硬枝扦插生根的影响及扦插生根与体内营养物质含量之间的关系,以1年生喜树枝条为材料,从基质种类、插穗部位、长度及粗度、ABT与NAA浓度等方面研究了不同处理对生根的影响,同时测定了喜树生根过程中可溶性糖、淀粉和蛋白质3种营养物质的含量。结果表明,泥炭土中插穗生根率最高,达96.67%; 选用1年生实生苗下部插穗的成活率最高,为97.33%; 粗度为0.6～0.9 cm、长度为10.0～15.0 cm的插穗,更利于生根。沙藏过的喜树插穗生根率高于不沙藏枝条。质量浓度为250 mg/L的ABT处理插穗效果最好,生根率达86.67%。硬枝扦插的喜树插穗是以皮部生根为主,兼有部分愈伤组织生根。生根过程中插穗基部的可溶性糖含量呈急剧下降后升高和再下降再上升的趋势,淀粉含量呈升高―降低―升高―降低的变化趋势,而蛋白质含量变化趋势与前两者都不一致。
The research aimed to explore the effects of different treatments on rooting of Camptotheca acuminata hardwood cutting, and the relationship between the content of nutrients and rooting of cuttings. One-year-old stems of C. acuminata was used as studying materials.The changes of soluble sugar, starch and soluble protein contents in cuttings during adventitious root formation were determined. The results were shown as follow: The rooting rate of cuttings in the peat soil was the highest with 96.67%. The one-year-old seedling in the lower part was selected, of which the highest rooting rate reached 97.33%. The optimal treatment for improving survival rate of C. acuminata was ABT 250 mg/L with the rooting rate of 86.67%.Cuttings with diameter of 0.6-0.9 cm and length of 10.0-15.0 cm were conducive to rooting, and the cutting rate stored in wet sand was higher. Roots were mainly induced from the bark and some roots were also developed from the callus tissues in hardwood cuttings. During the rooting process, the soluble sugar content in the base of cutting slips had a changing trend of sharply decreasing initially, then slowly increasing and decreasing again and finally increasing in the end. The starch content ascended initially and descended, then ascended again and descended at last. The changing trend of protein content varied from the former two