短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定

通过AB-8大孔吸附树脂对胆木药材水提液进行吸附，用不同比例的乙醇水溶液对其梯度洗脱，通过分离纯化获得纯度较高的短小舌根草苷单体。用UPLC-QTOF/MS分析技术鉴定大鼠灌胃短小舌根草苷后，尿液、粪便以及胆汁中该成分可能的代谢产物。采用全扫描方式采集所有成分准确MS/MS数据，利用MetaboLynx软件分析处理，获得了纯度较高的短小舌根草苷单体并鉴定出了其在大鼠体内的4种代谢产物。
In this paper, water extract of Nauclea officinalis was absorbed by AB-8 macroporous resin and subsequently gradient-eluted with alcoholic solution of different proportion to prepare pumiloside monomer of high purity. The metabolites in urine, feces and bile of rats with gavage administration were analyzed by UPLC-QTOF/MS. Accurate MS/MS data of all components were collected with full scan mode, and analyzed by MetaboLynx software. Results showed that the monomer of high-purity pumiloside was prepared and four metabolites in rats were identified