产中性纤维素酶细菌的筛选及培养基优化

从土壤中筛选到一株产中性纤维素酶的细菌,初步鉴定为芽孢杆菌属。以其为出发菌株,利用单因素及析因试验、爬坡实验、中心组合实验,建立其发酵产酶过程的响应面(RSM)模型,通过对RSM模型分析,得到最优培养基碳氮源组成:麦芽糖质量浓度1.97 g/dL、酵母粉-蛋白胨混合氮源(1∶4)2.03 g/dL。验证发现,优化后酶活从130.40 U/mL提高到194.23 U/mL。
A neutral cellulase-producting bacterium was screened from soil,and this strain was identified as Bacillus through 16S rDNA sequence analysis. With it as a starting strain,we constructed a response surface methodology(RSM) model by single factor and factorial test,ascent experiment and central composite experiments. By this RSM model,we obtained the optimal medium containing 1.97% maltose,2.03% yeast extract-tryptone(1∶4,m/m) mixed nitrogen. The enzyme activity increased from 130.40 U/mL to 194.23 U/mL under the optimal conditions