大豆皂苷I与II及苷元B对黄曲霉毒素B1和苯并芘突变效应的拮抗

本研究确认了大豆皂苷I与II及苷元B对黄曲霉毒素B1和苯并芘的抗突变作用。Ames试验中，除了最小剂量时大豆皂苷I与II未能显著抑制移码突变外，3个受试物均明显抑制黄曲霉毒素B1和苯并芘诱发的TA98和TA100回复突变。而且，除了最小剂量时大豆皂苷I与II未能显著抑制苯并芘与DNA的结合外，3个受试物均明显抑制人肝癌（HepG2）及支气管上皮（BEAS-2B）细胞中突变剂与DNA加合物的形成。大体上，苷元B比大豆皂苷I与II更有效地抑制移码与碱基置换突变和加合物的形成。因此，3个食源活性成分不仅抑制基因突变同时也拮抗突变剂诱导的DNA损害。
Inhibitory activities of soyasaponin I，II and their aglycone for aflatoxin B1（AFB1） and benzo[a]pyrene（B[a]P）-induced mutagenicity were investigated. In the Ames assay，Salmonella typhimurium strains TA98 and TA100 were selected for frameshift and base substitution mutations，respectively. Three compounds showed remarkable protection against AFB1 and B[a]P-induced mutations，with the exception that at the low concentration of 0.3 mg/plate soyasaponins I and II did not significantly inhibit the frameshift mutation. Three compounds also effectively protected against the formation of AFB1-DNA adduct in HepG2 cells and B[a]P-DNA adduct in BEAS-2B cells，wit