酵母双杂交法筛选组织蛋白酶B相互作用蛋白质

组织蛋白酶B是肿瘤侵袭和转移密切相关的一种蛋白酶，研究其作用网络对于开发基于组织蛋白酶B的抗肿瘤药物非常重要。采用酵母双杂交技术构建相关文库，以人组织蛋白酶B作为诱饵蛋白质，寻找其在肿瘤细胞中的直接相互作用蛋白质。在84个初始阳性克隆中，共有15个能通过3个报告基因的检测。BLAST分析发现，在GenBank数据库中可以匹配7个基因，编码7种已知的蛋白质：BCL2-associated athanogene 6（BAG6），　filaminA，alpha（FLNA），ras homolog family member G（RHOG），metallothionein 2A（MT2A），　ZXD family zinc finger C（ZXDC），DEP domain containing 7（DEPDC7），A kinase（PRKA） interacting protein 1（AKIP1）。
Cathepsin B is an enzymatic protein that was closely related with tumor cell invasion and metastasis. The network of Cathepsin B is very important for research and development of new anticancer drug，for example，new Cathepsin B inhibitor. Using yeast two hybrid technique，the proteins that interacting with Cathepsin B were identified. Results：After screening analysis，15 positive clones of putative interactions were obtained and they were identified by sequencing and utilizing of the data online（http：//www.ncbi.nlm.nih.gov）. There were 7 genes including BCL2-associated athanogene 6（BAG6），filamin A，alpha（FLNA），　ras homolog family member G（RHOG），　metallothionein 2A（MT2A），　ZXD family zinc finger C（ZXDC），　DEP domain containing 7（DEPDC7），A kinase（PRKA） interacting protein 1（AKIP1）