茶蜂花粉提取物BPE对LPS诱导的Raw 264.7细胞的体外抗炎症作用

以茶蜂花粉提取物（BPE）为实验材料，研究了其可能的抗炎活性。通过福林酚法和三氯化铝比色法测定BPE中的总酚酸和总黄酮质量分数，ABTS自由基清除实验评价BPE体外自由基清除能力，并采用LPS诱导的小鼠Raw 264.7细胞体外炎症模型，探究BPE对炎症因子和相关基因表达的调控作用。结果显示，BPE的总酚酸及总黄酮质量分数分别为（255.23±21.43） mg/g（绿原酸当量）和（132.85±14.77） mg/g（芦丁当量）；BPE的ABTS自由基清除力的IC50值为（53.9±5.38） μg/mL；在体外抗炎实验中，BPE能显著抑制Raw 264.7细胞NO的释放，显著抑制iNOS、IL-1β和IL-10，增强HO-1基因mRNA水平的表达，并呈一定的剂量相关性。表明，BPE富含黄酮和酚酸类物质并具有很强的自由基清除能力，在体外细胞实验中表现出了良好的抗炎症效果。
This study intended to evaluate the anti-inflammatory activity of Camellia sinensis bee pollen extract （BPE）. Total phenolic content and total flavonoid content of BPE were determined by the Folin-Ciocalteu method and AlCl3 colorimetry，respectively.Meanwhile， ABTS free-radical scavenging methods were used to evaluate its free-radical scavenging capacity. On the other hand，the anti-inflammatory effect of BPE was investigated in vitro by evaluating its modulating effects on the key inflammatory cytokines and the mRNA expression of inflammatory-mediators and cytokine genes in LPS stimulated Raw 264.7 cells. The results showed that the total phenolic content and total flavonoid content of BPE were 255.23±21.43 mg/g and 132.85±14.77 mg/g，respectively. BPE exhibited strong ABTS free-radical scavenging capacity and its IC50 value was 53.9±5.38 μg/mL. In vitro，BPE exhibited significantly to inhibit NO release and the mRNA expression of iNOS，IL-1β and IL-10，and to increase the mRNA expression of HO-1. The results indicated BPE was rich in phenolic compounds and had strong free-radical scavenging capacity，and showed a good anti-inflammatory effects in vitro