MALDI-TOF-MS鉴定3种水稻细菌的方法

用哥伦比亚培养基培养了水稻细菌性谷枯病菌（NCPPB 2391，NCPPB 3591）、水稻细菌性条斑病菌（NCPPB 1585）、水稻细菌性褐斑病菌（NCPPB 2844），菌落采用乙醇/甲酸处理法处理后用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析其蛋白质指纹图谱。除水稻细菌性条斑病菌匹配结果外，其它2种细菌均有正确的鉴定结果。用Bruker公司标准方法建立了水稻细菌性条斑病菌蛋白质指纹图谱库。采用水稻叶片作为空白样品添加了上述3种植物病菌，回收实验结果表明3种植物病菌均可以用本方法检测。该研究为应用MALDI-TOF-MS方法检测水稻细菌奠定了基础。
After Burkholderia glumae (NCPPB 2391, NCPPB 3591), Xanthomonas oryzae pv. Oryzicola (NCPPB 1585) and Pseudomonas syringae pv. Syringae (NCPPB2844) were cultured by Columbia agar medium and bacterial colonies were treated with an ethanol-formic acid extraction procedure, the protein fingerprints were obtained and analyzed by MALDI-TOF-MS. Three plant pathogens were accurately identified except that Pseudomonas syringae pv. Syringae did not exist in the Bio-Typer. With the standard method from Bruker, we established the protein fingerprint of Pseudomonas syringae pv. syringae in Bio-Typer. For the rice leaf spiked with these rice pathogens