造纸法再造烟叶涂布液中腐败菌的分离鉴定与抑菌

采用平板划线分离方法对造纸法再造烟叶涂布液中的主要微生物群进行分离，再根据细菌的菌落形态、菌体形态、生理生化实验和16S rDNA序列分析对菌株进行了鉴定。结果表明，鉴定出引起涂布液腐败的细菌为短小芽孢杆菌、地衣芽孢杆菌和肺炎克雷伯菌，其中肺炎克雷伯菌为主要腐败菌。此外，还考察了山梨酸钾、双乙酸钠、尼泊金酯钠、乙二胺四乙酸二钠和ε-聚赖氨酸等5种防腐剂对肺炎克雷伯菌的抑菌效果。结果表明，5种防腐剂的抑菌效果强弱顺序为ε-聚赖氨酸>乙二胺四乙酸二钠>双乙酸钠>山梨酸钾>尼泊金酯钠，其中ε-聚赖氨酸对肺炎克雷伯菌的最小抑菌质量浓度为0.075 mg/mL。
In this paper，the streak plate separation method was used to classify and identify the primary bacterial flora in the coating solution of paper-making reconstituted tobacco sheet according to colony shape，bacterial characteristics，physio-biochemical tests as well as 16S rDNA sequence analysis. The results indicated that the spoilage bacteria in the coating solution of paper-making reconstituted tobacco sheet were Bacillus pumilus，Bacillus licheniformis and Klebsiella pneumonia. Among them，Klebsiella pneumoniae was the main spoilage bacteria. Moreover，the antibacterial effects of five food preservatives[potassium sorbate，sodium diacetate，butylparaben sodium，EDTA-2Na and ε-polylysine（ε-PL）] on the spoilage bacteria were evaluated. The results revealed that antibacterial activities of these five preservatives ranked as follows：ε-PL> EDTA-2Na > sodium diacetate > potassium sorbate > butylparaben sodium. The minimal inhibitory concentration of ε-PL on Klebsiella pneumoniae was 0.075 mg/mL