海洋细菌Planococcus rifietoensis发酵产色素培养基的优化

在单因素的基础上，以Plackett-Burman（PB）设计结合响应面（RSM）分析法，对产色素海洋细菌Planococcus rifietoensis发酵培养基的8个营养成分配比进行优化。结果表明：培养基最佳碳氮源分别是葡萄糖和蛋白胨，葡萄糖、MgSO4·7H2O和酵母粉添加量显著影响色素的产量。Box-Benhnken设计分析确定最优培养基配方为：葡萄糖9.78 g/L，蛋白胨 8.00 g/L，酵母粉2.05 g/L，MgSO4·7H2O 8.17 g/L，Na2HPO4 0.1 g/L，FeSO4·7H2O 0.005 g/L，NaCl 10 g/L，CaCl2 0.1 g/L，培养54 h，在此培养条件下，色素的OD值可达0.984，比优化前提高了245%。
Based on monofactorial experiments，Plackett-Burman（PB） design and response surface methodology（RSM） were applied to optimize 8 medium components for the production of pigments by marine bacteria strain Planococcus rifietoensis. Results showed that optimal carbon and nitrogen sources of the medium were glucose and peptone，respectively，and glucose，yeast extract and MgSO4·7H2O had major effects on the production of pigments. The optimal culture medium was comprised of glucose 9.78 g/L，peptone 8.00 g/L，yeast extract 2.05 g/L，MgSO4·7H2O 8.17 g/L，Na2HPO4 0.1 g/L，FeSO4·7H2O 0.005 g/L，NaCl 10 g/L and CaCl2 0.1 g/L. The incubation time was 54 hours. Under these conditions，the optical density（OD） of pigments reached to 0.984，which was 245% higher than that before optimization