连续微通氧浓醪乙醇发酵研究

为了提高浓醪酒精发酵效率，以高浓度木薯粉浆液化滤液为原料，对微通氧浓醪酒精发酵过程进行了研究。在搅拌式5 L发酵罐中，通过对连续通风速率分别为0、0.04、0.08、0.16、0.24、0.35 L/min的高浓度液化滤液酒精分批发酵过程分析表明，最佳通风策略为：以通风速率0.08 L/min进行酒精发酵，酵母细胞密度达到5.40×108个/mL，发酵效率为90.00%，与未通风发酵相比分别提高了56.52%和2.45%。总之，在浓醪酒精发酵过程中微通氧能够提高酵母细胞活性，进而提高发酵效率。
Ethanol is widely used in renewable fuels，chemical engineering，medicine and food industry. In developed countries there is a growing trend towards employing modern technologies and ef?cient bio-energy conversion using a range of biofuels. In order to improve Very High Gravity（VHG） ethanol fermentation efficiency the optimization process of VHG ethanol fermentation from liquefied VHG cassava mash filtrate with micro-aeration were investigated in this thesis. Changes of fermentation parameters during the ethanol fermentation from VHG liquefied starch filtrate with aeration rate （0，0.04，0.08，0.16，0.24，0.35 L/min） by Saccharomyces cerevisiae GGSF16 under batch condition in a 5-L bioreactor were studied. The optimum condition for ethanol fermentation was shown as a continuous aeration rate of 0.08 L/min. The verification experiments under the optimum condition clearly indicated that the aeration strategies improved ethanol production. The biomass and fermentation efficiency were 5.40×108 cells/mL and 90.00%，respectively，representing about 56.52% and 2.45% increase compared to anaeration fermentation. In conclusion，micro-aeration can improve VHG ethanol fermentation efficiency because it can enhance cell viability