SOD乳酸菌高产菌株筛选鉴定及SOD分离纯化

筛选出一株产SOD乳酸菌，并对SOD进行分离纯化。采用平板富集、革兰氏染色等方法进行初筛。再通过邻苯三酚自氧化法检测SOD酶活进行复筛。并经形态学、生理生化试验、16S-rDNA基因序列分析进行菌种鉴定。通过硫酸铵沉淀、超滤、Sephadex-G100、DEAE-Sepharose FF等方法进行纯化。筛选得到一株产SOD相对较高菌株SD006，经鉴定为Lactobacillus plantarum，纯化后酶活达37 959.2 U/g。细菌SD006是一株产SOD的植物乳酸菌，乳酸菌是一种人体益生菌，乳酸菌SOD可作为食用SOD添加到食品中，具有潜在开发价值和广阔应用前景。
One SOD high-producing strain was screened and identified from lactic acid bacteria. The SOD enzyme was purified. The microbial enrichment，Gram staining and Pyrogallol autoxidation determination method were used in the screening. Morphological characteristics，physiology and biochemistry experiments，16S-rDNA sequencing were used for the strain identification. The SOD enzyme was purified by ammonium sulfate precipitation，ultrafiltration，sephadex-G100，DEAE-Sepharose FF etc. A SOD high-producing strain （SD006） was obtained and identified as Lactobacillus plantrum. The activity of purified enzyme reached 37 959.2 U/g SD006 is a SOD high-producing probiotics strain of Lactobacillus plantrum. The SOD purified from Lactobacillus is valuable and can be developed and applied in the food industry