CdTe量子点与微囊藻毒素-LR抗体的偶联及其相互作用的研究

以巯基乙酸作为稳定剂制备了水溶性CdTe量子点，将纯化后的量子点与微囊藻毒素-LR抗体偶联，通过分析紫外-可见吸收光谱和荧光光谱，研究了不同温度下CdTe量子点与微囊藻毒素-LR抗体的相互作用，得到了量子点与抗体之间的表观结合常数Kapp（84.3M-1），Stern-Volmer猝灭常数KSV（6.74×104（298 K），6.11×104（304 K），5.93×104（310 K）），结合位点n和结合常数K。此外，还研究了量子点与抗体之间相互作用的热力学参数△H（＜0），△G（＜0）和△S（＞0），经实验数据发现，量子点与抗体之间的作用力以静电相互作用为主，氢键、范德华力也起到了一定的作用。
Water-soluble CdTe quantum dots（QDs） were synthesized using thioglycollic acid as a stabilizer，and the purified QDs were bind to the Microcystin-LR antibody. The interaction between the QDs and the Microcystin-LR antibody at different temperatures were investigated. The association constant Kapp（84.3M-1），the Stern-Volmer quenching constant Ksv（6.74×104（298 K），6.11×104（304 K），5.93×104（310 K）），the number of binding sites n，and the binding constant K were all calculated through the analysis of UV-Vis absorption and fluorescence spectrum. In addition，the interaction of the thermodynamic parameters between the QDs and the antibody were studied ，△H（＜0），△G（＜0）and △S（＞0），indicating that electrostatic played mainly roles in the interactions between CdTe QDs and Microcystin-LR antibody，and the hydrogen bond and van der Waals also made the contributions