新型黑曲霉液体发酵及对黄曲霉毒素B1的脱除

对经紫外照射诱变制备的新型黑曲霉FS-UV1在马铃薯葡萄糖液体培养基（PDB）中的pH、蛋白质质量浓度、蛋白酶活性变化以及脱除机制进行了研究。在发酵过程中，pH值不断下降，由初始pH 5.9下降为1.83。蛋白质质量浓度在发酵48 h后达到最大，为10.07 g/L，而酸性蛋白酶活性则在72 h达到最高，为0.98 U/mL。新型黑曲霉FS-UV1孢子悬液对AFB1脱除效果，而菌丝体对AFB1具有吸附作用，发酵液则对AFB1具有降解作用，其中发挥降解作用的可能是蛋白质。对FS-UV1在pH 7的模拟肠道环境中脱除黄曲霉毒素B1（AFB1）的效果进行了评价。脱除48 h时，在pH 7的模拟肠道环境中对AFB1的脱除率为87.3%。
In this paper，the changes in pH，protein，protease activity and the degradation mechanism of the new Aspergillus niger FS - UV1 which was induced by ultraviolet irradiation in potato glucose liquid medium（PDB） were studied. It is found that the pH value is declining in fermentation process，which dropped from the initial 5.9 to 1.83. The protein content showed the maximum of 10.07 g/L at 48 h，and protein activity reached the highest at 72 h with 0.98 U/ mL. The experimental results showed that AFB1 could be decontaminated by mycelium and culture filtrate，and the spores had effect on AFB1. In addition， FS - UV1 degraded aflatoxin B1（AFB1） effect was evaluated in simulation intestinal environment of pH= 7 and the degradation rate was 87.3%