分光光度法检测大豆乳清废水中大豆异黄酮浓度

以实际排放的大豆乳清废水作为研究体系，在研究乙醇浓度、体积、水浴 温度和超声时间对检测大豆异黄酮浓度的影响基础上，提出将超声波与高温水浴 结合的方法以消除蛋白质对检测造成的干扰．采用响应面分析方法，确定了最佳 的检测条件为水浴温度85 ℃、超声功率200 W和超声时间30 min，测得此大豆乳 清废水中大豆异黄酮的浓度为0.367 g/L，与高效液相色谱测得的结果0.380 g/L 相比，相对误差仅为3.4%．该方法具有仪器简单和快速检测的优点，可用于工业 生产中测定废水体系中大豆异黄酮的浓度．
In order to eliminate the interference caused by the protein s for measuring soy isoflavone concentration, a method of ultrasound w ave coupling with high-temperature bath was developed using the direct discharged soy whey wastewater as actual system on the basis of the s tudies of the effects of ethanol concentration, volume, bath temperatu re and ultrasound time on the soy isoflavone concentration. Response s urface methodology was used to determine the optimal measure condition s: bath temperature 85 ℃, ultrasonic power 200 W and ultrasound time 30 min. The soy isoflavone concentration in the wastewater was 0.367 g /L and the relative error was only 3.4% compared with the result of 0. 380 g/L measured by high performance liquid chromatography. The method has the advantages of simple instrument and fast measurement and thus , it can be used in industrial production to measure the soy isoflavon e concentration of the wastewaters.