藏红花素对小鼠电点燃癫痫的影响

观察藏红花素对小鼠颞叶癫痫形成过程和大发作的影响。 利用C57小鼠海马电点燃癫痫模型，观察藏红花素对癫痫形成过程中的发作等级、累积后放电时间、在各等级停留的刺激次数、动物最终停留阶段等指标的影响，以及其对癫痫大发作动物的大发作概率、平均发作等级、后放电持续时间等指标的影响。 与溶剂对照组比较，20 mg/kg藏红花素能抑制小鼠海马电点燃癫痫形成过程的每日平均发作等级（P < 0.05），缩短每日累积后放电持续时间（P < 0.01），同时延长癫痫在1~2级停留的刺激次数（P < 0.05），并降低小鼠完全点燃的比例（P < 0.01）。10或50 mg/kg藏红花素对上述指标均无明显影响（P＞0.05）。对于已经完全点燃的动物，100或200 mg/kg藏红花素均能降低其大发作的概率（均P < 0.01），降低平均发作等级（均P < 0.01）；50 mg/kg藏红花素则仅对平均发作等级有降低作用（P < 0.05）。 藏红花素在小鼠海马电点燃模型中能抑制癫痫形成过程和癫痫大发作，其效果呈现一定的剂量依赖性，可能是一种有开发和应用前景的潜在抗癫痫药物。
Abstract: Objective To investigate the effect of crocin on the progression and generalized seizure of temporal lobe epilepsy in mice. Methods Hippocampus rapid kindling model was established in C57BL/6J mice. The effects of crocin on seizure stage, afterdischarge duration (ADD), number of stimulation in each stage and final state, the incidence of generalized seizure (GS), average seizure stage and ADD were observed. Results Crocin (20 mg/kg) significantly retarded behavioral seizure stages (P < 0.05) and shortened cumulative ADD (P < 0.01) during hippocampus rapid kindling acquisition in mice compared with vehicle group. Meanwhile, number of stimulations in stage 1-2 was significantly increased (P < 0.05) and the incidence of fully kindled animals was significantly decreased (P < 0.01). However, 10 or 50 mg/kg crocin showed no significant effect on the above indexes (all P>0.05). Crocin (100 or 200 mg/kg) significantly decreased the incidence of GS (all P < 0.01) and reduced average seizure stages (all P < 0.01) in fully-kindled mice compared with vehicle group; Fifty mg/kg crocin only reduced average seizure stages (P < 0.05). Conclusion Low-dose crocin can retard the progression in hippocampus rapid kindling acquisition in mice, while high-dose crocin relieves the GS in fully-kindled mice, which suggests that crocin may be a potential anti-epileptic compound. Key words: Epilepsy, temporal lobe PICROCROCIN/therapeutic use PICROCROCIN/administration&dosage Kndling, neurologic/drug effects Dose-response relationship, drug Disease models, animal