锦花清热胶囊抗内毒素作用实验研究

探讨锦花清热胶囊的解热和抗内毒素作用。 选健康雄性新西兰兔30只，采用注射脂多糖法建模，然后将所有新西兰兔分为五组：模型对照组（等渗氯化钠溶液），阳性对照组（阿司匹林0.2 g/kg），锦花清热胶囊大、中、小剂量组（分别含生药6.0、3.0、1.5 g/kg），每组6只，观察锦花清热胶囊对内毒素所致发热新西兰兔的降温作用；取昆明小鼠50只，分为五组：模型对照组（等渗氯化钠溶液），阳性对照组（阿司匹林0.2 g/kg），锦花清热胶囊大、中、小剂量组（分别含生药6.0、3.0、1.5 g/kg），每组10只，采用显色基质法检测锦花清热胶囊对昆明小鼠血浆中内毒素的降解率。 新西兰兔解热实验结果显示，锦花清热胶囊大、中剂量组在60 min后降温作用显著，其中大剂量组在300 min时已经接近基础体温；小剂量组在180 min后开始有降温作用，提示锦花清热胶囊对发热新西兰兔有降温作用；昆明小鼠抗内毒素实验结果显示，锦花清热胶囊大、中、小剂量组在30 min时内毒素的降解率为（56.73±3.12）%、（47.23±1.77）%、（21.08±2.30）%，60 min时为（82.76±1.00）%、（64.75±1.77）%、（38.21±1.57）%，提示锦花清热胶囊对昆明小鼠有降低内毒素的作用。 锦花清热胶囊有显著的解热和抗内毒素作用，可以为临床治疗热毒类疾病提供依据。
Abstract: Objective To investigate the anti-pyretic and anti-endotoxin effect of Chinese herb medicine Jinhuaqingre capsules. Methods Thirty healthy male New Zealand rabbits with lipopolysaccharide-induced fever were divided into 5 groups (6 rabbits in each): animals in model group were given normal saline by gavage, animals in positive control group were given aspirin (0.2 g/kg), and animals in Jinhuaqingre groups were given Jinhuaqingre capsules 6.0, 3.0 or 1.5 g/kg, respectively. The changes in body temperature of rabbits were observed. Fifty healthy Kunming mice were divided into 5 groups (10 mice in each): mice in model group were given normal saline by gavage, mice in positive control group were given aspirin (0.2 g/kg), and those in Jinhuaqingre groups were given Jinhuaqingre capsules 6.0, 3.0, 1.5 g/kg, respectively. Matrix coloration method was used to detect the degradation rate of endotoxin in mice. Results The body temperature in rabbits of high and medium dose Jinhuaqingre capsule groups declined significantly 60 min after drug administration, and the temperature of high-dose group returned to the baseline after 300 min; while the body temperature of low-dose group started to decline at 180 min after drug administration. The endotoxin degradation rates in mice of high, medium and low dose groups was (56.73±3.12)%, (47.23±1.77)% and (21.08±2.30)% at 30 min after drug administration; those were (82.76±1.00)%, (64.75±1.77)% and (38.21±1.57)% at 60 min after drug administration, respectively. Conclusion Chinese herb medicine Jinhuanigre capsules have anti-pyretic and anti-endotoxin effects, which may provide a new option for the treatment of heat-toxin syndrome. Key words: Fever/traditional Chinese medicine therapy Disease models, animal Endotoxins/adverse effects Antipyretic agents/therapeutic use Lipopolysaccharides