低盐饮食对犬类心脏组织基因表达的影响

目的：探讨低盐饮食对哺乳动物心脏组织基因表达谱的影响，并分析差异表达基因可能涉及的分子生物学功能。方法：从基因芯片公共数据库（GEO）中获取低盐饮食心脏组织基因芯片数据（GSE17149），将数据导入Qlucore Omics Explorer 3.1、STRING 10.0、Genclip 2.0、GCBI等分析软件，筛选低盐饮食组与正常饮食组实验动物心脏组织差异表达基因，并分析差异表达基因编码蛋白质相互作用网络等，筛选两组之间的关键节点基因。结果：与正常饮食组比较，低盐饮食组犬心脏组织基因表达谱发生改变，在43 035个基因中共有1343个（3.12%）差异表达基因；差异表达基因蛋白质相互作用网络分析发现1个最明显的蛋白质互相作用网络，以NF-κB抑制因子-α（NFKBIA）蛋白为核心，主要发挥减弱巨噬细胞源性泡沫细胞分化、促进胆固醇排出和分解等生物学功能。结论：低盐饮食动物心脏组织基因转录谱发生明显改变，低盐饮食可能通过上调NFKBIA基因的表达来降低心血管疾病的风险。
Abstract: Objective:To investigate the effect of low-salt diet on gene expression of canine cardiac tissue. Methods:Microarray data (GSE17149) of heart tissue from dogs fed with low-salt diet was obtained from the Gene Expression Omnibus (GEO). The data were analyzed by Qlucore Omics Explorer 3.1, STRING 10.0, Genclip 2.0 and GCBI. The protein-protein interactions (PPI) of the differentially expressed genes in low-salt and control groups were conducted to screen the key node genes between the two groups. Results:Compared with the control group, the gene expression profile of the dog heart tissue was changed in the low-salt diet group, and 1343 (3.12%) differentially expressed genes were found in 43 035 genes. The differentially expressed gene protein interaction analysis revealed a most obvious protein (NFKBIA) as the core, which might be related to inhibiting the differentiation of macrophage-derived foam cell and promoting cholesterol discharge and decomposition. Conclusion:Gene expression of heart tissue in dogs fed with low-salt diet is significantly changed and low-salt diet may reduce the risk of cardiovascular disease by up-regulating the expression of NFKBIA. Key words: Diet, sodium-restricted Cardiovascular diseases/prevention & control Gene expression Atherosclerosis/physiopathology Transcription, genetic Mass spectrometry Computational biology Cells, cultured