神经元特异性烯醇化酶和突触素在人胚胎食管组织发育阶段的表达

目的:探讨神经元特异性烯醇化酶(NSE)和突触素(SYN)蛋白在人胚胎食管组织不同发育阶段的分布特征。方法:经伦理委员会批准,收集受精龄为7～16周的人胚胎标本16例,应用免疫组织化学染色法检测胚胎食管组织内NSE和SYN的表达,定量分析其变化规律。结果:第2～4个月胎龄时,肌间神经丛和黏膜下神经丛内细胞呈NSE阳性表达,阳性表达的数量和强度第3个月胎龄甚于第2个月胎龄,第4个月胎龄甚于第3个月胎龄。仅在第3个月胎龄时,食管黏膜上皮层内部分细胞呈NSE阳性表达。第2～4个月胎龄时,SYN在食管各层组织内均呈广泛阳性或强阳性表达,尤其在肌间神经丛和黏膜下神经丛更为明显。结论:在人胚胎食管发育阶段, NSE和SYN均参与食管壁神经系统的发育过程。
Abstract: Objective: To investigate the expression of neuron-specific enolase (NSE) and synaptophysin(SYN) proteins in different developmental stages of human embryonic esophagus. Methods: Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age (n=16). One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age. Results: In fetuses with 2, 3 and 4 months of gestation, the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38±8.37, 25.00±11.54 and 38.00±15.09, respectively; the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38±14.93, 62.25±18.59 and 56.44±14.70, respectively. NSE-positive cells were detected in the esophageal epithelium only at the third month. In the fetuses at 2, 3 and 4 months of gestation, SYN in all layers of esophageal tissue were positively or strong positively expressed, especially in the myenteric plexus and submucosal plexus. The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2, 3 and 4 month gestation were 54.69±9.34, 51.84±6.10 and 46.41±6.44, respectively. Conclusion: SYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development. Key words: Esophagus/patholoy Esophagus/embryology Phosphopyruvate hydratase Synapsins Immunohistochemistry