4种非生物胁迫下小麦幼苗表型及可溶性蛋白含量的变化
Changes of phenotype and soluble protein content in wheat seedlings under four kinds of abiotic stress

对小麦幼苗分别进行低温(4℃)、高温(42℃)、高盐(800 mM NaCl)及干旱(20% PEG)4种非生物胁迫，从麦苗生长表型及其根、茎、叶片总可溶性蛋白含量的变化方面分析不同胁迫对小麦幼苗生长及生理变化的影响。结果表明：经过4种胁迫处理后，小麦幼苗生长均处于不同程度的抑制状态，高温、高盐和干旱胁迫下植株干枯明显，低温下植株明显较对照矮小；高温胁迫后幼苗根长仅5 cm左右，比正常植株短8～9 cm，高盐、干旱及低温条件下植株根长比正常植株短4.83 cm；4种胁迫下叶片长度也明显小于正常植株。胁迫直接导致了小麦根、茎和叶中可溶性蛋白含量不同程度的增加，根中总蛋白含量较茎和叶中低，根对胁迫也最为敏感，高温胁迫12 h后，根中总蛋白含量达到最大3.92 μg·mg-1，高温胁迫24 h后，茎中总蛋白含量接近15 μg·mg-1，而叶片中总蛋白的含量却保持72 h持续增长的状态。
The changes in phenotype and total soluble protein content were determined of roots, stems and leaves of wheat seedlings by exposing to different abiotic stresses, such as low temperature (4℃), high temperature (42℃), high salinity (800 mM NaCl) and drought (20% PEG). Stresses caused inhibition in growth at different extent. Desiccation of plants occurred due to high temperature, high salinity and drought whereas low temperature resulted undersized individuals. The average length of root was about 5 cm which was 8～9 cm shorter than the wild type under high temperature. On the contrary, it is 6 cm shorter when treated with low temperature, high salinity and drought. Moreover, the total soluble protein content of experimental parts increased due to stresses. According to results, root was sensitive to stresses and it lad much lower protein content than stems and leaves. The protein content of root, stem reached 3.918 μg·mg-1 and 15 μg·mg-1 for high temperature at 12 h and 24 h, respectively, while in leaves it kept increasing till 72 h.