外源硫化氢对加工番茄种子耐盐性及抗氧化酶的影响
Effects of exogenous hydrogen sulfide on salt tolerance and antioxidant enzymes of processing tomato seeds

为明确硫化氢对盐胁迫下加工番茄种子萌发的缓解作用，用NaCl半致死浓度为85 mmol·L-1的KT-63(耐盐性弱)和半致死浓度为120 mmol·L-1的KT-18(耐盐性强)为材料，用各自的半致死浓度与不同浓度的NaHS(0.2，0.4，0.6，1.0 mmol·L-1)分别配成混合液处理种子。结果表明，随着NaHS浓度的增加，种子的发芽率、发芽势、发芽指数、活力指数、SOD、POD、CAT活性呈先上升后下降的趋势，其中与盐胁迫单独处理相比，用0.4 mmol·L-1 NaHS处理，种子的发芽率、发芽势、发芽指数、活力指数、SOD、POD、CAT活性在KT-63中分别提升70%、250%、120%、130%、6.7%、150%和180%，在KT-18中分别提升50%、250%、60%、80%、4.3%、160%和200%，而MDA的含量显著降低，并且对KT-63的缓解效应强于KT-18。综上所述，H2S(0.4 mmol·L-1)有效缓解了加工番茄萌发过程中盐胁迫的抑制作用，促进了种子的萌发。
The effects of exogenous hydrogen sulfide on the seed germination of processing tomato under salt stress were investigated. Seeds of two processing tomato cultivars KT-63(salt resistance is weak) and KT-18(stronger salt tolerance) were treatment with semi-lethal concentration of NaCl(85 mmol·L-1, 120 mmol·L-1) and NaHS(0.2,0.4,0.6,1.0 mmol·L-1) mixture, respectively. The results showed that: With increasing NaHS level, the germination rate, germination energy, germination index, vigor index, SOD, POD and CAT activities first increased and then decreased. Compared with salt stress, treatments with 0.4 mmol·L-1 NaHS significantly increased seed germination rate, germination index, vigor index and the activities of SOD, POD and CAT in KT-63(70%,250%,120%,130%,6.7%,150% and 180%, respectively) and KT-18(50%,250%,60%,80%,4.3%,160% and 200%, respectively), and the content of MDA was decreased. The mitigation effect of hydrogen sulfide on KT-63 is stronger than KT-18. Results provide evidence that 0.4 mmol·L-1 of H2S may alleviate damage to seeds caused by salt stress, expedition of seed germination.