CONSTANS-LIKE 7基因对拟南芥开花的调控分析

为研究CONSTANS-LIKE 7 (COL7)在调控植物开花方面的功能，以定量PCR,GUS染色等方法，研究光及生物钟对COL7表达的影响，以及COL7对拟南芥开花的影响.实验结果显示：光及生物钟参与调控COL7的表达；过表达COL7在长日照条件下抑制CONSTANS (CO)以及FLOWERING LOCUS T (FT)的表达，进而抑制拟南芥开花；col7突变体不论是在长日照下还是短日照下都没有明显的开花表型，说明COL7在调控拟南芥开化方面可能与其家族基因中的其它成员存在功能冗余.
To discover the function of CONSTANS-LIKE 7 (COL7) in regulating plant flowering, we used qPCR, GUS staining, et al, to study the light and circadian regulating the expression of COL7, and COL7 regulating Arabidopsis flowering time. The results showed that COL7 was regulated by light and circadian, and the overexpressing COL7 transgenic lines inhibited Arabidopsis flowering time under long-day (LD) condition through down-regulating CONSTANS (CO) and FLOWERING LOCUS T (FT), and the col7 mutant had no obvious flowering phenotype under LD or SD conditions. So COL7 may have redundant functions with other CONSTANS-LIKEs (COLs) in controlling the flowering time of Arabidopsis.