C/N对好氧反硝化菌强化的SBR脱氮效率的影响

摘要 使用2株具有良好的好氧反硝化能力的假单胞菌对序批式反应器(SBR)进行生物强化，连续运行61 d，探讨进水C/N变化对微生物强化SBR内微生物菌群和其脱氮效率的影响.随着C/N降低，反硝化效率明显降低，但是经强化的SBR的反硝化效率始终优于对照组，向活性污泥中接种好氧反硝化细菌后，NO-3-N 去除率最高提升14.1%，使用纯菌构建人工菌群可将NO-3-N去除率提高22.6%.使用好氧反硝化菌进行强化可以提升反硝化细菌所占比例，促使其迅速成为优势功能菌群，缩短系统的启动周期，大幅提高系统的反硝化效率，并且提高菌群结构和系统运行的稳定性.该技术在处理C/N>10∶1的含硝酸盐的污水时更具优势.
Abstract：Two strains of Pseudomonas with efficient aerobic denitrification ability were used in the bio-augmentation process of sequencing batch reactor. In the 61 days’ operating process， the effects of C/N on the microbial community structures and denitrification efficiency were investigated. Although the denitrification efficiencies obviously decreased with the C/N decreasing， the performances of bio-augmentation groups were better than those of the negative control. Adding efficient aerobic denitrifiers into activated sludge enhanced the NO-3-N removal rate by about 14.1%， whereas the pure-cultured group was enhanced by 22.6%. Bio-augmentation with aerobic denitrifiers enhanced the proportion of denitrifiers， promoted them to be the dominant functional population， shortened the system’s start-up period， greatly enhanced the denitrification efficiency， and improved the stability of the microbial community structure and the system’s operation. This technique is more advantageous in the treatment of C/N>10∶1 wastewater containing nitrate.