8种猕猴桃抗氧化活性评价及基于HPLC与FT-IR指纹分析的品种区分

为探明不同品种猕猴桃的体外抗氧化活性差异，并对不同猕猴桃品种进行有效区分，采用高效液相色谱法（HPLC）、傅里叶变换红外光谱法（FT-IR）对猕猴桃进行指纹分析，分析测定了脐红、金艳等8个品种猕猴桃的体外抗氧化能力、多酚组成及FT-IR光谱，并对其进行了方差分析、主成分分析（PCA）及系统聚类分析（CA）。结果表明：华优的抗坏血酸及总酚含量比其他品种高约2倍，其体外抗氧化活性显著强于其他品种。对抗氧化指标、酚类含量及FT-IR数据的主成分分析中前两个主成分累积解释率分别达到63.49%、55.91%、84.96%。三种聚类分析均可将不同品种猕猴桃进行较好区分，尤其对金艳、华优、海沃德区分效果最好。基于FT-IR数据的品种区分效果优于基于抗氧化指标、酚类含量的区分，同植物学分类最为一致。基于抗氧化指标的聚类分析对脐红、晚红、徐香的区分效果欠佳。
To analyze the antioxidant capacity of different kiwi fruit cultivars and to distinguish between different kiwi cultivars, high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR) were employed to conduct fingerprint analysis for kiwi fruit samples. The antioxidant capacity, phenolic content, and FT-IR spectra of Qihong, Jinyan, and six other kiwi fruit cultivars were measured, and analysis of variance, principal component analysis (PCA), and hierarchical cluster analysis (CA) were conducted. The results showed that the content of ascorbic acid and total phenols in Huayou were about twice as high as those found in other cultivars, and Huayou had the strongest in vitro antioxidant capacity. The cumulative proportions of explained variance for the first two principal components from the PCA of antioxidant indices, polyphenolic content, and FI-IR data were 63.49%, 55.91%, and 84.96%, respectively. Three cluster analyses could effectively distinguish between the different cultivars; Jinyan, Huayou, and Hayward were best separated. The separation of cultivars based on FT-IR spectroscopic analysis was better than separation based on antioxidant indexes and phenolic content, and showed a result most similar to botanical classification. The separation using CA based on antioxidant indexes was not effective enough for the Qihong, Wanhong, and Xuxiang cultivars.