光照胁迫对雨生红球藻虾青素积累和抗氧化活性的影响

本文研究了不同光强(120~270 μmol/m2?s)和光周期（光/暗：0/24~24/0 h）的光照胁迫对雨生红球藻(Haematococcus pluvialis)虾青素积累、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、总抗氧化能力（T-AOC）和超氧阴离子自由基（O2-?）清除能力的影响。结果表明，在绿色、褐色和红色细胞期，光强越高，藻细胞虾青素的含量、T-AOC和O2-?清除能力也越高；抗氧化酶（SOD和CAT）活性在绿色细胞期随光强的增加而增加，在红色细胞期则随光强的增加而下降；藻细胞虾青素的含量、T-AOC和O2-?清除能力在红色细胞期最高，而抗氧化酶活性在褐色细胞期最高；藻细胞在不同光周期的270 μmol/m2?s的光照下生长10 d时，虾青素的含量、T-AOC和O2-?清除能力随每日光照时间的延长而增高，抗氧化酶活性则先升高后下降。总之，雨生红球藻在270 μmol/m2?s的光强下连续光照10 d时虾青素的含量和抗氧化能力最高，这为虾青素作为天然的抗氧化功能食品添加剂的生产和应用提供了新的参考条件。
The effects of light stress with different light intensities (120~270 μmol/m2?s) and photoperiods (light/dark: 0/24-24/0 h) on the astaxanthin accumulation, superoxide dismutase (SOD) and catalase (CAT) activities, total antioxidant capacity (T-AOC), and superoxide anion free radical (O2-?) scavenging ability of Haematococcus pluvialis were examined. The results showed that at green, brown, and red cell stages, a higher light intensity led to higher astaxanthin content and greater T-AOC and O2-? scavenging ability. The activities of the antioxidant enzymes (SOD and CAT) increased at the green cell stage and decreased at the red cell stage, with increasing light intensity. The highest astaxanthin content and greatest T-AOC and O2-? scavenging ability were obtained at the red cell stage, whereas the highest antioxidant enzyme activities were obtained at the brown cell stage. when H. pluvialis grew for ten days under different photoperiods and a light intensity of 270 μmol/m2?s, the astaxanthin content and T-AOC and O2-? scavenging ability increased with the extensions of the daily illumination period, whereas the activities of the antioxidant enzymes decreased after the initial increase. In summary, H. pluvialis showed the highest astaxanthin content and antioxidant capacity after continuous illumination at a light intensity of 270 μmol/m2?s for ten days. This finding provides a new reference for the production and application of astaxanthin as a natural antioxidant food additive.