五味子抑制α-葡萄糖苷酶活性成分的研究

本研究探索了五味子中总木脂素、总黄酮、总三萜及五味子醇乙、五味子甲素、五味子醇甲、五味子酯甲对α-葡萄糖苷酶活性的抑制作用。利用体外α-葡萄糖苷酶活性抑制模型，以4-硝基苯-α-D-吡喃葡萄糖苷(PNPG)为底物，阿卡波糖(ACAR)为阳性对照，测定了五味子中总木脂素、总黄酮、总三萜及五味子醇乙、五味子甲素、五味子醇甲、五味子酯甲对α-葡萄糖苷酶活性的体外抑制作用。五味子中总木脂素、总黄酮、总三萜浓度为1.0 mg/mL对α-葡萄糖苷酶抑制率分别为96.15±1.52%、60.33±1.84%和48.62±2.15%，五味子醇乙、五味子甲素、五味子醇甲和五味子酯甲浓度为0.33 mg/mL对α-葡萄糖苷酶活性的抑制率分别为96.53±1.62%、68.48±1.83%、15.57±2.37%和6.24±2.49%。五味子中总木脂素及五味子醇乙均对α-葡萄糖苷酶活性有显著抑制作用，且具有明显的量效关系。
The inhibitory effects of total lignans, total flavone, total triterpenes, schisandrol B, deoxyschizandrin, schisandrin and schisantherin A of Fructus schisandrae on the α-glucosidase activity. Using the in vitro inhibition of α-glucosidase activity as a model and 4-nitrophenyl α-D-glucopyranoside (PNPG) as substrate as well as acarbose (ACAR) as a positive control, the inhibitory effects of total lignans, total flavone, total triterpenes, schisandrol B, deoxyschizandrin, schisandrin and schisantherin A of Fructus schisandrae on the activity of α-glycosidase were determined in vitro. When the concentrations of total lignan, total flavone and total triterpenes in Fructus schisandrae were 1.0 mg/mL, the inhibitory rates of α-glucosidase were 96.15±1.52%, 60.33±1.84% and 48.62±2.15%, respectively. When the concentrations of schisandrol B, deoxyschizandrin, schisandrin and schisantherin A were of 0.33 mg/mL, the inhibitory rates of α-glucosidase were 96.53±1.62%, 68.48±1.83%, 15.57±2.37%, 6.24±2.49%, respectively. The total lignans and schisandrin B in Fructus schisandrae had a significant inhibitory effect on the activity of α-glucosidase, and had a significant dose-effect relationship.