高糖环境对滋养层细胞腺苷单磷酸活化蛋白激酶 α1 亚基表达及增殖的影响
Influence of high glucose on PRKAA1 expression and proliferation of trophoblast cells

目的 · 研究妊娠糖尿病患者胎盘组织中的腺苷单磷酸活化蛋白激酶 α1 亚基（protein kinase AMP-activated catalytic subunit α1， PRKAA1）的表达水平，及体外高糖环境对滋养层细胞 PRKAA1 表达水平和增殖活性的影响。方法 · 收集妊娠糖尿病患者（19 例） 及同期正常妊娠孕妇（20 例）的胎盘组织，分别利用实时荧光定量 PCR 及蛋白免疫印迹试验测定 PRKAA1 mRNA 及蛋白水平的表达 情况。体外高糖处理滋养层细胞后，测定 PRKAA1 的表达；高糖培养基及 PRKAA1 抑制剂 dorsomorphin 分别作用于滋养层细胞后， 利用 CCK8 试剂盒测定细胞增殖活性。结果 · 相比正常妊娠孕妇，妊娠糖尿病患者胎盘组织中 PRKAA1 mRNA 及蛋白表达量显著降低 ( 均 P<0.05)。体外高糖培养基处理滋养层细胞可明显降低 PRKAA1 的表达水平 (P<0.05)；高糖培养基和 dorsomorphin 均可抑制滋养 层细胞的增殖活性 ( 均 P<0.05)。结论 · 妊娠糖尿病患者胎盘组织中的 PRKAA1 表达水平下降，体外高糖环境对滋养层细胞增殖活性 的抑制作用可能是通过下调 PRKAA1 介导发生。
：Objective · To explore the expression level of protein kinase AMP-activated catalytic subunit α1 (PRKAA1) in placental tissues of gestational diabetes mellitus (GDM) women, and the influence of high glucose (HG) on PRKAA1 expression and proliferation viability of trophoblast cells in vitro. Methods · The placental samples of GDM women (n=19) and normal pregnant women (n=20) of the corresponding period were collected. Real-time qPCR and Western blotting assay were used to detect the mRNA and protein levels of PRKAA1 in these biopsies, respectively. Trophoblast cells were treated by HG in vitro and then expression level of PRKAA1 was tested. CCK8 assay was used to detect proliferation viability of the cells treated by HG medium or inhibitor of PRKAA1, dorsomorphin. Results · Comparing to normal pregnant women, both mRNA and protein levels of PRKAA1 in placental tissues of GDM women significantly decreased (both P<0.05). HG treatment drastically downregulated expression of PRKAA1 in trophoblast cells in vitro (P<0.05). Both HG medium and dorsomorphin suppressed proliferation viability of trophoblast cells (both P<0.05). Conclusion · Expression level of PRKAA1 is dampened in placental tissues of GDM women. HG suppresses proliferation viability of trophoblast cells probably via downregulating PRKAA1 level in vitro