孕妇尿酚类环境内分泌干扰物浓度检测的尿样收集方法比较
Urine sample collection methods for measuring urinary concentrations of phenolic endocrine disrupting chemicals and their comparison in pregnant women

目的 · 探讨孕妇尿酚类环境内分泌干扰物检测的尿样收集方法，采用并比较2 种方法对尿双酚A（BPA）、三氯生（TCS） 与正壬基酚（4-n-NP）检测浓度的影响。方法 · 采用2 种方法收集孕晚期尿样：导尿管尿袋收集（n=176），聚丙烯（PP）尿杯收 集（n=642）。采用高效液相色谱– 串联质谱(HPLC-MS/MS) 法检测尿BPA、TCS 及 4-n-NP 的浓度。验证实验采用PP 材质尿杯、 导尿管尿袋、低密度聚乙烯材质离心管及玻璃杯（阴性对照）留取同一妇女共 5 人的尿样。结果 · 导尿管尿袋收集的尿样BPA 浓 度几何均数为82.5 ng/mL（95% CI 为 71.4 ～ 95.4 ng/mL），比尿杯留取尿样的BPA 浓度高63 倍（几何均数1.3 ng/mL，95% CI 为 1.3 ～ 1.5 ng/mL）； 2 种方法尿 TCS、4-n-NP 及肌酐浓度相似。验证实验的结果表明，相同个体的尿样，尿袋收集的尿 BPA 浓度远远 高于其他 3 种方法。结论 · 尿酚类环境内分泌干扰物浓度检测的尿样收集，用 PP 材质尿杯优于导尿管尿袋收集，PP 材质尿杯及低 密度聚乙烯材质试管适合于流行病学人群研究。
： Objective · To compare urine sample collection methods for measuring urinary concentrations of phenolic endocrine disrupting chemicals including bisphenol A (BPA), triclosan (TCS), and 4-n-nonylphenol (4-n-NP) in pregnant women. Methods · Urine samples were collected from women at late pregnancy by two methods urine catheter and collection bag (n=176), urine collecting containers made of polypropylene (PP) (n=642). Urinary concentrations of BPA, TCS and 4-n-NP were measured with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. Confirmation experiment used PP containers, urine catheter and collection bags, as well as low-density polyethylene (LDPE) tubes and glass containers as both negative controls to collect urine samples from each of the 5 women. Results · Geometric mean (GM) of urinary BPA concentration collected by urine catheter and collection bags was 82.5 ng/mL (95% CI 71.4-95.4 ng/mL), which was 63 times higher than that from PP containers (GM 1.3 ng/mL; 95% CI 1.3-1.5 ng/mL). Concentrations of urinary 4-n-NP and creatinine were similar between two collection methods. Confirmation experiment showed that urinary BPA concentration collected by urine catheter and collection bags was much higher than those collected by other three methods. Conclusion · In collection of urine samples for measuring phenolic chemicals, PP urine collection container as well as LDPE containers are adequate for use in epidemiologic studies, but urine catheter and collection bag is not