超高效液相色谱- 四极杆飞行时间质谱联用技术分析人类唾液代谢组的性别依赖性
Gender dependence of metabolomic phenotypes for human saliva using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry

目的 · 研究人类唾液代谢组的性别依赖性。方法 · 使用基于超高效液相色谱 – 四极杆飞行时间质谱（UHPLC-Q-TOFMS）联 用的代谢组学技术，检测分析年龄、体质量指数、生活及洁牙条件匹配的 5 位男性与 5 位女性健康志愿者的唾液样本代谢物组成，再 通过与已有数据库对比和标准品验证鉴定唾液中的代谢物群，确定 2 组样品中差异具有统计学意义的代谢物。结果 · 鉴定了人类唾液 中的13 种氨基酸、6 种胆碱代谢物、15 种肉碱类代谢物、4 种鞘氨醇类代谢物、7 种溶血磷脂酰胆碱和3 种有机酸等48 种代谢物。 发现苯丙氨酸、乙酰基肉碱、丙酰基肉碱、丁酰基肉碱、异丁酰基肉碱、异戊酰基肉碱和鞘氨醇的含量在女性唾液中显著高于男性样 本。结论 · 人类唾液中含有丰富的代谢物信息，唾液代谢组研究必须考虑其性别依赖性。
：Objective · To investigate the gender-related metabolomic differences in human saliva. Methods · The saliva metabolomic profiles of 5 male and 5 female healthy volunteers with matched age, body mass index (BMI), living and tooth-brushing condition were acquired using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Metabolites were identified using publicly accessible databases and further confirmed individually with standard compounds. Both multivariant and univariate statistics were conducted to find inter-gender differences. Results · Forty-eight metabolites in human saliva were identified including 13 amino acids, 6 choline metabolites, 15 carnitines, 4 sphinganine metabolites, 7 lysophosphatidylcholine and 3 organic acids. Levels of phenylalanine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isobutyrylcarnitine, isovalerylcarnitine and sphinganine were higher in the saliva of females than that of males. Conclusion · Rich metabolic information present in human saliva with significant gender diffference which ought to be taken into consideration in study on the metabonomics of saliva