黄酮类化合物影响大麻素受体过表达细胞中 cAMP 含量的初步研究
Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells#br#

目的 · 初步探讨黄酮类化合物对大麻素受体（CBR）过表达细胞中环磷酸腺苷（cAMP）含量的影响。方法 · 构建重组表达质 粒 GV144-CB1R 和 GV144-CB2R；转染 HEK-293 细胞建立 CBR 过表达细胞模型，研究黄酮类化合物对 CBR 过表达细胞中 cAMP 含 量的影响。结果 · 成功构建 CBR 过表达细胞模型。化合物作用结果显示，所测试的化合物在测试浓度下均可使过表达CBR 的 HEK293 细胞中 cAMP 含量显著降低，且对 CB2R 过表达的细胞有一定选择性。结论 · 该研究所使用的黄酮类化合物都可以在 μmol/L 浓度 下降低 CBR 过表达细胞中 cAMP 的含量。
：Objective · To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells. Methods · Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK293 cell lines. Results · The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells. Conclusion · All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level