三氯生对人早孕滋养细胞11β-HSD2和HLA-G表达的影响
Effects of triclosan on expression of 11β-HSD2 and HLA-G in human first trimester trophoblasts

目的 探讨三氯生对人早孕滋养细胞11β-羟类固醇脱氢酶2（11β-HSD2）和人类白细胞分化抗原G（HLA-G）表达的影响。方法 采用改良的Kliman法分离提纯早孕滋养细胞，分离得到的滋养细胞包括绒毛细胞滋养细胞和绒毛外细胞滋养细胞，绒毛细胞滋养细胞体外培养的过程中合体化形成合体滋养细胞，培养3 d后，以不同浓度的三氯生（0、0.0001、0.001、0.01、0.1、1 μmol/L）染毒处理早孕滋养细胞24 h，Western blotting检测合体滋养细胞11β-HSD2的表达及绒毛外细胞滋养细胞HLA-G的表达，明胶酶谱实验检测绒毛外细胞滋养细胞基质金属蛋白酶2/9(MMP2/9)的酶活性。结果 经三氯生染毒处理后，合体滋养细胞中11β-HSD2的表达及绒毛外细胞滋养细胞中HLA-G的表达均显著减少，绒毛外细胞滋养细胞MMP2/9的酶活性降低。结论 一定浓度的三氯生可以抑制人早孕滋养细胞11β-HSD2和HLA-G的表达及MMP2/9的酶活性。提示过量暴露三氯生可能对女性生殖健康产生不利影响。
： Objective To investigate the effects of triclosan on expression of 11β-HSD2 and HLA-G in human first trimester trophoblasts. Methods First trimester trophoblasts were isolated and purified with modified Kliman method, including villous cytotrophoblasts and extravillous cytotrophoblasts. Villous cytotrophoblasts fused to form syncytiotrophoblasts during culture in vitro. First trimester trophoblasts were treated with different concentrations of triclosan (0, 0.000 1, 0.001, 0.01, 0.1, and 1 μmol/L) for 24 h after three days of culture. The expression of 11β-HSD2 in syncytiotrophoblasts and HLA-G in extravillous cytotrophoblasts was detected by Western blotting. The gel zymography was used to detect the enzymatic activity of MMP2/9 in extravillous cytotrophoblasts. Results The expression of 11β-HSD2 in syncytiotrophoblasts and HLA-G in extravillous cytotrophoblasts was significantly decreased and the enzymatic activity of MMP2/9 in extravillous cytotrophoblasts was reduced after triclosan treatment. Conclusion Triclosan can inhibit the expression of 11β-HSD2 and HLA-G and the enzymatic activity of MMP2/9 in human first trimester trophoblasts, suggesting excessive exposure to triclosan may have adverse effects on the reproductive health in females