细胞黏附的微吸管法测定及其应用进展
Application progresses in measuring cell adhesion by micropipette aspiration assay

细胞及亚细胞结构之间的黏附参与细胞迁移、增殖、分化、免疫激活、肿瘤转移等重要的病理生理过程。结合细胞生物学、免疫学和生物力学等交叉学科研究方法，定量测定细胞黏附力的大小，并建立相应的动力学数学模型，可以进一步阐明细胞黏附的特征，为深入解析淋巴细胞在免疫应答中的相互作用模式、药物对载体细胞的靶向性、生物材料与组织细胞的兼容程度、肿瘤细胞转移的生物力学机制等提供新的理论依据和干预策略。该文介绍微吸管法测定细胞黏附的基本原理，并对该方法的应用进展进行综述。
： The adhesion between cells or subcellular structures participates in important pathophysiological processes such as cell migration, proliferation, differentiation, immune activation and tumor metastasis. Cross-discipline research methods can integrate cell biology, immunology, and biomechanics, and quantitatively measure the cell adhesion force. With the establishment of corresponding dynamic mathematical models, biophysical properties of cell adhesion can be further elucidated. Thus novel theoretic evidence and intervention strategies can be provided for in-depth understanding of interaction models of lymphocytes during immune response, targeting of drugs towards carrier cells, compatibility between biomaterials and tissue cells, as well as biomechanical mechanism of tumor metastasis. This article introduces the principles of micropipette aspiration assay for measuring cell adhesion and reviews its latest applications