LRIG3蛋白在宫颈鳞癌中的表达及与细胞增殖的关系
Protein expression of LRIG3 in tissues of cervical squamous cell carcinoma and its relation with cell proliferation

目的 探讨LRIG3蛋白在宫颈鳞癌组织中的表达及与细胞增殖的关系。方法 收集郑州大学第一附属医院病理科2009—2011年石蜡包埋标本宫颈鳞状细胞癌45例、宫颈上皮内瘤变（CIN）标本20例、正常宫颈组织30例，采用免疫组织化学SP法，检测宫颈组织中LRIG3及Ki-67蛋白的表达，进行统计学分析。结果 LRIG3蛋白在宫颈鳞癌、CIN及正常宫颈组织中的阳性表达率分别为35.56%、70.00%、100.00%，且差异均有统计学意义(P<0.05)；Ki-67蛋白在宫颈鳞癌组织、CIN组织以及正常宫颈黏膜组织中的表达阳性率为86.67%、30.00%、3.33%，3组间比较差异有统计学意义（P<0.01）。LRIG3表达与Ki-67表达呈显著相关性（P<0.01）。结论 LRIG3蛋白在宫颈鳞癌中呈低表达，与肿瘤细胞增殖相关，提示LRIG3下调可能与宫颈鳞癌有关。
： Objective To explore the protein expression of LRIG3 in tissues of cervical squamous cell carcinoma and the correlation between the protein expression of LRIG3 and cell proliferation. Methods The paraffin embedded specimens of 45 cases of cervical squamous cell carcinoma, 20 cases of cervical intraepithelial neoplasia (CIN), and 30 cases of normal cervical tissues from 2009 to 2011 were collected from the Pathology Department of the First Affiliated Hospital of Zhengzhou University. Immunohistochemical SP method was adopted to detect the protein expressions of LRIG3 and Ki-67 in cervical tissues and statistical analysis was performed. Results The positive expression rates of LRIG3 in tissues of cervical squamous cell carcinoma and CIN and normal cervical tissues were 35.56%, 70.00%, and 100.00%, respectively and the differences were statistically significant (P<0.05). The positive expression rates of Ki-67 in tissues of cervical squamous cell carcinoma and CIN and normal cervical tissues were 86.67%, 30.00%, and 3.33%, respectively and the differences were statistically significant (P<0.01). The expression of LRIG3 significantly correlated with the expression of Ki-67 (P<0.01). Conclusion The low protein expression of LRIG3 in tissues of cervical squamous cell carcinoma is relevant to the proliferation of tumor cells, which suggests the down-regulation of LRIG3 may be relevant to cervical squamous cell carcinoma