超声辐照对兔足跖炎性痛模型组织前列腺素E₂的影响
Effects of ultrasound irradiation on prostaglandins E₂ in inflammatory tissue of rabbit paw

目的 探索低强度超声辐照对炎症组织前列腺素E2的影响。 方法 24只新西兰大白兔分为超声辐照足跖组、超声辐照股二头肌组、假照组和正常组。除正常组外，其他各组兔足跖注射角叉菜胶建立炎性痛模型，超声辐照足跖组、超声辐照股二头肌组分别予0.2 MHz超声辐照足跖、同侧股二头肌处20 min，假照组予假辐照。分别于辐照后即刻和1 h采耳中动脉血，ELISA法检测β-内啡肽水平；第二次采血结束后，取材双侧后腿足跖10 mg，ELISA法检测前列腺素E2。 结果 超声辐照足跖组的前列腺素E2浓度显著高于超声辐照股二头肌组、假照组和正常组，超声辐照足跖组的血浆β-内啡肽浓度与超声辐照股二头肌组相比在两个检测时间点均无统计差异。 结论 低强度超声辐照股二头肌也能提高血浆β-内啡肽水平，且不会促进炎症组织前列腺素释放，是超声辐照缓解炎性痛的更优治疗方案。
： Objective To investigate the effects of low-intensity ultrasound irradiation on prostaglandins E2 in inflammatory tissues. Methods Twenty-four New Zealand rabbits were randomly divided into the ultrasound irradiation paw group, ultrasound irradiation biceps femoris group, pseudo irradiation group, and normal control group. Except the normal control group, paws of rabbits of other groups were injected with carrageenan solution to establish the inflammatory pain model. Paws of rabbits of the ultrasound irradiation paw group and ipsilateral biceps femoris of rabbits of the ultrasound irradiation biceps femoris group were irradiated with 0.2 MHz ultrasound for 20 min. Rabbits of the pseudo irradiation group were ultrasound were irradiated without power for 20 min. The blood samples were collected from ear artery immediately and 1 h after irradiation. The level of β-endorphin was detected by the ELISA. After the second blood sampling, 10 mg of paw tissue of both hind legs were collected and the level of prostaglandin E2 was detected by the ELISA. Results The level of prostaglandin E2 of the ultrasound irradiation paw group was significantly higher than those of the ultrasound irradiation biceps femoris group, pseudo irradiation group, and the normal control group. The differences of serum levels of β-endorphin of the ultrasound irradiation paw group and ultrasound irradiation biceps femoris group at two time points were not statistically significant. Conclusion Low-intensity ultrasound irradiation of biceps femoris can improve the serum level of β-endorphin and will not promote the release of prostaglandin E2 in inflammation tissues. It is an ideal method for relieving the inflammatory pain by ultrasound irradiation